README: Touch sensation requires the mechanically-gated ion channel ELKIN1.

https://doi.org/10.5061/dryad.0cfxpnw8s

The dataset uploaded here is in prism format and contains all the figures shown in Chakrabarti et al 2023. Datasets were derived from a variety of experimental protocols including behavioral data, patch clamp electrophysiology, skin nerve electrophysiology, immunohistochemistry, tripartite-GFP assay. All the data here are processed, along with corresponding graphs.

Description of the data and file structure

Data are structured in a prism format according to the figure numbers of the manuscript. For example, if the name of the prism file is "Fig2_Supp3", it contains data and graphs from Figure 2 and Supplementary Figure Fig S3 from the manuscript. The names of the data tables in prism is descriptive of the experiment conducted followed by the technique used. For example, the data table named "Threshold_patchclamp_pillar" contains data of thresholds of mechanical activation as assessed using pillar assay and patch clamp electrophysiology. All methods are explained the Supplementary file of the manuscript.

All statistical analysis is also provided in the Results tab and graphs in the Graphs tab of the prism files. Listed here are the files uploaded and the description of the relevant experiments:

Fig1_Suppl1_upload.pzfx: Data from Fig 1 and Fig S1 - all data from patch clamp electrophysiology.

Fig2_Suppl3_upload.pzfx: Data from Fig 2 and Fig S3 - contains behavioral and immunohistochemical read out

Fig3_Suppl4_Suppl5_upload.pzfx: Data from Fig 3, Fig S4 and Fig S5 - contains patch clamp electrophysiology and immunocytochemistry data

Fig4_Suppl8_upload.pzfx: Data from Fig 4, Fig S8 - all data from skin nerve electrophysiology

Fig5_Suppl9_Suppl10_upload.pzfx: Data from Fig 5, Fig S9 and FIg S10 - contains patch clamp electrophysiology and skin nerve electrophysiology data

Suppmelentary2_ SC.pzfx: Data from Fig S2 - contains cell attached patch clamp data with pressure clamp

Supplementary6_SC.pzfx: Data from Fig S6 - all data from patch clamp electrophysiology with poking of human-derived sensory neurons

Supplementary7_SC.pzfx: Data from Fig S7- contains triGFP experiments and patch clamp data showing interaction between STOML3 and ELKIN1. Statistics are also provided.