Touch sensation requires the mechanically gated ion channel ELKIN1
Data files
Feb 15, 2024 version files 3.53 MB
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Fig1_Suppl1_upload.pzfx
195.09 KB
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Fig2_Suppl3_upload.pzfx
869.29 KB
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Fig3_Suppl4_Suppl5_upload.pzfx
224.16 KB
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Fig4_Suppl8_upload.pzfx
140.17 KB
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Fig5_Suppl9_Suppl10_upload.pzfx
139.37 KB
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README.md
2.33 KB
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Supplementary2_SC.pzfx
1.64 MB
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Supplementary6_SC.pzfx
236.72 KB
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Supplementary7_SC.pzfx
88.41 KB
Feb 18, 2024 version files 3.53 MB
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Fig1_Suppl1_upload.pzfx
195.09 KB
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Fig2_Suppl3_upload.pzfx
869.29 KB
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Fig3_Suppl4_Suppl5_upload.pzfx
224.16 KB
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Fig4_Suppl8_upload.pzfx
140.17 KB
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Fig5_Suppl9_Suppl10_upload.pzfx
139.37 KB
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README.md
2.33 KB
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Supplementary2_SC.pzfx
1.64 MB
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Supplementary6_SC.pzfx
236.72 KB
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Supplementary7_SC.pzfx
88.41 KB
Abstract
The extraordinary speed of touch perception is enabled by mechanically-activated ion channels, the opening of which excites cutaneous sensory endings to initiate sensation. We identify Elkin1(1) as an ion channel likely gated by mechanical force necessary for normal behavioral touch sensitivity in mice. Touch insensitivity in Elkin1-/- mice was caused by a loss of mechanically-activated currents (MA-currents) in around half of all sensory neurons that are activated by light touch (low threshold mechanoreceptors, LTMRs). Reintroduction of Elkin1 into sensory neurons from Elkin1-/- mice acutely restored MA-currents. Additionally, siRNA mediated knockdown of Elkin1 from induced human sensory neurons substantially reduced indentation-induced MA-currents supporting a conserved role for Elkin1 in human touch. Our data identify Elkin1 as a novel core component of touch transduction in mammals.
README: Touch sensation requires the mechanically-gated ion channel ELKIN1.
https://doi.org/10.5061/dryad.0cfxpnw8s
The dataset uploaded here is in prism format and contains all the figures shown in Chakrabarti et al 2023. Datasets were derived from a variety of experimental protocols including behavioral data, patch clamp electrophysiology, skin nerve electrophysiology, immunohistochemistry, tripartite-GFP assay. All the data here are processed, along with corresponding graphs.
Description of the data and file structure
Data are structured in a prism format according to the figure numbers of the manuscript. For example, if the name of the prism file is "Fig2_Supp3", it contains data and graphs from Figure 2 and Supplementary Figure Fig S3 from the manuscript. The names of the data tables in prism is descriptive of the experiment conducted followed by the technique used. For example, the data table named "Threshold_patchclamp_pillar" contains data of thresholds of mechanical activation as assessed using pillar assay and patch clamp electrophysiology. All methods are explained the Supplementary file of the manuscript.
All statistical analysis is also provided in the Results tab and graphs in the Graphs tab of the prism files. Listed here are the files uploaded and the description of the relevant experiments:
Fig1_Suppl1_upload.pzfx: Data from Fig 1 and Fig S1 - all data from patch clamp electrophysiology.
Fig2_Suppl3_upload.pzfx: Data from Fig 2 and Fig S3 - contains behavioral and immunohistochemical read out
Fig3_Suppl4_Suppl5_upload.pzfx: Data from Fig 3, Fig S4 and Fig S5 - contains patch clamp electrophysiology and immunocytochemistry data
Fig4_Suppl8_upload.pzfx: Data from Fig 4, Fig S8 - all data from skin nerve electrophysiology
Fig5_Suppl9_Suppl10_upload.pzfx: Data from Fig 5, Fig S9 and FIg S10 - contains patch clamp electrophysiology and skin nerve electrophysiology data
Suppmelentary2_ SC.pzfx: Data from Fig S2 - contains cell attached patch clamp data with pressure clamp
Supplementary6_SC.pzfx: Data from Fig S6 - all data from patch clamp electrophysiology with poking of human-derived sensory neurons
Supplementary7_SC.pzfx: Data from Fig S7- contains triGFP experiments and patch clamp data showing interaction between STOML3 and ELKIN1. Statistics are also provided.