Data from: Divergence in style length and pollen size leads to a postmating-prezygotic reproductive barrier among populations of Silene latifolia
Brothers, Amanda N., Indiana University Bloomington
Delph, Lynda F., Indiana University Bloomington
Published Mar 28, 2017 on Dryad.
Cite this dataset
Brothers, Amanda N.; Delph, Lynda F. (2017). Data from: Divergence in style length and pollen size leads to a postmating-prezygotic reproductive barrier among populations of Silene latifolia [Dataset]. Dryad. https://doi.org/10.5061/dryad.0f8j8
A central tenet of speciation research is the need to identify reproductive isolating barriers. One approach to this line of research is to identify the phenotypes that lead to reproductive isolation. Several studies on flowering plants have shown that differences in style length contribute to reproductive isolation between species, leading us to consider whether style length could act as a reproductive barrier among populations of a single species. This could occur if style length varied sufficiently and pollen size covaried with style length. Populations of Silene latifolia exhibit variation in flower size, including style length, that is negatively correlated with annual precipitation. We show that this divergence in style length has a genetic basis and acts as a reproductive barrier: males from small-flowered populations produced relatively small pollen grains that were poor at fertilizing ovules when crossed to females from large-flowered populations, leading to a significant reduction in seed production. Manipulating the distance pollen tubes had to travel revealed that this failure was purely mechanical and not the result of other incompatibilities. These results show that style length acts as a postmating-prezygotic reproductive barrier and indicate a potential link between ecotypic differentiation and reproductive isolation within a species.
Flower size data
Flower size data
style length correlates
Data on style length, abiotic measures, and petal size for the six study populations.
Data on reproductive isolation
Data for each female from the six populations for the Reproductive Isolation measure, number of seeds produced from base pollinations, and whether the male was from the same or a different population as the individual female.
pollen grain style length correlation
Data on pollen grain size and style length for individuals from the six study populations.
location of pollen deposition
Data of measurements of where pollen grains were deposited on styles in nature.
location of ovules fertilized
Data on the number of ovules fertilized when the male was from the same population as the female or from separate small-flowered populations.