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Dryad

Environmentally acquired gut-associated bacteria are not critical for growth and survival in a solitary bee, Megachile rotundata

Cite this dataset

Brar, Gagandeep (2024). Environmentally acquired gut-associated bacteria are not critical for growth and survival in a solitary bee, Megachile rotundata [Dataset]. Dryad. https://doi.org/10.5061/dryad.0gb5mkm90

Abstract

Social bees have been extensively studied for their gut microbial functions, but the significance of the gut microbiota in solitary bees remain less explored. Solitary bee, Megachile rotundata females provision their offspring with pollen from various plant species, harboring a diverse microbial community that colonizes larvae guts.​ The Apilactobacillus is the most abundant microbe, but evidence concerning the effects of Apilactobacillus and ​other provision microbes on growth and survival ​are ​lacking. We ​hypothesized that the presence of Apilactobacillus in abundance would enhance larval and prepupal development, weight, and survival, while the absence of intact microbial communities was expected to have a negative impact on bee fitness. We reared larvae on pollen provisions with naturally collected microbial communities (Natural pollen) or devoid of microbial communities (Sterile pollen). We also assessed the impact of introducing Apilactobacillus micheneri by adding it to both types of pollen provisions. Feeding larvae with sterile pollen + A. micheneri led to the highest mortality rate, followed by natural pollen + A. micheneri, and sterile pollen. Larval development was significantly delayed in groups fed with sterile pollen. Interestingly, larval and prepupal weights did not significantly differ across treatments compared to natural pollen-fed larvae. 16S rRNA gene sequencing found a dominance of Sodalis, when A. micheneri was introduced to natural pollen. The presence of Sodalis with abundant A. micheneri suggests potential crosstalk between both, shaping bee nutrition and health. Hence, this study highlights that the reliance on non-host specific environmental bacteria may not impact fitness of M. rotundata.

README: Microbiome Megachile rotundata

https://doi.org/10.5061/dryad.0gb5mkm90

Legends in the data: (N), natural pollen with added A. micheneri (NAm), Sterile pollen (S), sterile pollen with A. micheneri (Sam), and natural pollen with an antibacterial cocktail (AC).

All .qzv file must be downloaded and uploaded to https://view.qiime2.org/  

16Staxabarplots_clean2.qzv : Relative abundances of the bacteria presented in Megachile rotundata larvae after feeding on treated pollen. Treatments were: natural pollen (N), natural pollen with added A. micheneri (NL), Sterile pollen (S), sterile pollen with A. micheneri (SL), and natural pollen with an antibacterial cocktail (AB).

demux-full_(2).qzv file represent Demultiplexed sequence counts summary

stats-dada2.qzv file represents filtered, denoised, merged and non chimeric reads

16s_MAPs_GB.tsv represents barcode sequences used for sequencing

alpha_rarefaction3.qzv represents alpha rarefaction- sequences 

Funding

National Science Foundation, Award: Track-2 FEC 1826834

National Science Foundation, Award: IOS-1557940

United States Department of Agriculture, Award: 3060-21220-032-00D