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Data for: Altered Circadian Rhythm, Sleep, and Rhodopsin 7-Dependent Shade Preference During Diapause in Drosophila Melanogaster

Cite this dataset

Montell, Denise et al. (2024). Data for: Altered Circadian Rhythm, Sleep, and Rhodopsin 7-Dependent Shade Preference During Diapause in Drosophila Melanogaster [Dataset]. Dryad. https://doi.org/10.5061/dryad.0p2ngf28n

Abstract

To survive adverse environments, many animals enter a dormant state such as hibernation, dauer, or diapause. Various Drosophila species undergo adult reproductive diapause in response to cool temperatures and/or short day-length. While it is known that flies are less active during diapause, an in-depth understanding of diapause effects on circadian rhythms and sleep is lacking. Here we show that, in diapause-inducing conditions, Drosophila melanogaster exhibit altered circadian activity profiles, including a severely reduced morning activity peak and an advanced evening activity peak. Consequently, the flies have a single activity peak at a time similar to when non-diapausing flies have a siesta. Temperatures ≤15 °C, rather than short day-length, primarily drive the behavior. At cool temperatures, flies also rapidly enter a deep sleep state that lacks the sleep cycles of flies at higher temperatures and requires particularly high levels of stimulation for arousal. Furthermore, we show that at 18–25 °C, flies prefer to siesta in the shade, a preference that is virtually eliminated at 10 °C. Resting in the shade is driven by an aversion to blue light, sensed by rhodopsin 7 (Rh7) outside of the eyes. Flies at 10 ˚C show neuronal markers of elevated sleep pressure, including increased expression of Bruchpilot and elevated Ca2+ in the R5 ellipsoid body neurons. Therefore, sleep pressure might overcome blue light aversion. Thus at temperatures known to cause reproductive arrest, preserve germline stem cells, and extend lifespan, Drosophila melanogaster are prone to deep sleep and exhibit dramatically altered - yet rhythmic - daily activity patterns.

README: Data for: Altered Circadian Rhythm, Sleep, and Rhodopsin 7-Dependent Shade Preference During Diapause in Drosophila Melanogaster

https://doi.org/10.5061/dryad.0p2ngf28n

Enclosed are the source data for the article "Altered circadian rhythm, sleep, and rhodopsin 7-dependent shade preference during diapause in Drosophila melanogaster."

Each figure has a separate .xlsx file, with the source data for each panel provided on a separate sheet (unless indicated otherwise). Due to the large array size in Figure 3d, we have included these data in a separate MATLAB file, "Figure_3d_StimSpeedTable.mat." 

Image data associated with Figure 6 and SI Figure 4 are saved as .czi files, which are binned in zip files.

The zip file names containing raw image data and their corresponding figures are as follows:

  • Figure 6a: Figure_6a.zip
  • Figure 6c: Figure_6c.zip
  • SI Figure 4 c and d: SI_Fig4_C_D.zip

Code/Software

GitHub repository: https://github.com/Craig-Montell-Lab/Meyerhof-et-al.-2024-

Methods

Refer to the linked manuscript ("Altered circadian rhythm, sleep, and rhodopsin 7-dependent shade preference during diapause in Drosophila melanogaster".

Funding

National Institute on Aging, Award: R01AG36907

National Institute of Allergy and Infectious Diseases, Award: R01AI169386