Data from: Using a comprehensive DNA barcode library to detect novel egg and larval host plant associations in a Cephaloleia Rolled-leaf Beetle (Coleoptera: Chrysomelidae)
Garcia-Robledo, Carlos, National Museum of Natural History
Kuprewicz, Erin K., National Museum of Natural History
Staines, Charles L., Smithsonian Institution
Kress, W. John, Smithsonian Institution
Erwin, Terry L., Smithsonian Institution
Published Apr 29, 2013 on Dryad.
Cite this dataset
Garcia-Robledo, Carlos et al. (2013). Data from: Using a comprehensive DNA barcode library to detect novel egg and larval host plant associations in a Cephaloleia Rolled-leaf Beetle (Coleoptera: Chrysomelidae) [Dataset]. Dryad. https://doi.org/10.5061/dryad.11p01
To fully understand the ecology and evolution of plant-herbivore interactions, information regarding the life history of both immature and adult insect stages is essential. However, most knowledge of plant-herbivore associations is derived from observations of adults. One reason for this bias is that species identification of immature stages is usually challenging. DNA barcodes can be used to identify immature stages to the species-level. This technique compares short sequences of the appropriate DNA barcode loci (e.g., mitochondrial COI gene for insects) of an unidentified specimen to a known DNA barcode library. The accuracy of DNA-based identifications depends on the comprehensiveness of the DNA barcode library. We generated a comprehensive DNA barcode library for a community of Rolled-leaf Beetles (Coleoptera: Chrysomelidae) in a premontane tropical forest in Costa Rica. The DNA barcode COI accurately identified all beetle species included in this study. Using this DNA barcode library, we identified eggs and larvae of Cephaloleia histrionica Baly with 100% confidence. This new record of C. histrionica is unique in that this species completes its life cycle on a bromeliad, whereas most Cephaloleia species are associated with plants from the order Zingiberales. The life cycle, diet breadth, immature stages, and sexual dimorphism are described for C. histrionica.
Samples included in DNA barcode analyses
Supplement S1. Samples included in DNA barcode analyses. Tables summarize collection information, the life stage of each sample (egg, larva or adult), host plant family / species and sequence quality (percentage of untrimmed bases of high-quality, HQ%). DNA amplifications were performed with the Folmer primers: GGTCAACAAATCATAAAGATATTGG (F) and TAAACTTCAGGGTGACCAAAAAATCA (R).
DNA sequences (COI) of limited quality and not deposited in GenBank (FASTA format).
Supplement S2. DNA sequences (COI) of limited quality and not deposited in GenBank (FASTA format). Sequences in this supplement were included in the analyses as they successfully identified individuals to the species level with 100% confidence. Numbers at the beginning of each sequence represent collection numbers. Information for each collection was included in Supplement S1.