Data from: The contributions of sex, genotype and age to transcriptional variance in Drosophila melanogaster
Data files
Mar 28, 2008 version files 89.02 MB
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CloneLUT.xls
1.18 MB
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MFCS10a.DAT
790.57 KB
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MFCS10b.DAT
802.83 KB
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MFCS11a.DAT
793.56 KB
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MFCS11b.DAT
790.04 KB
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MFCS12a2.DAT
793.13 KB
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MFCS15a2.DAT
797.39 KB
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MFCS15b2.DAT
790.42 KB
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MFCS16a2.DAT
800.30 KB
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MFCS1a.DAT
780.66 KB
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MFCS1b.DAT
788.27 KB
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MFCS3a.DAT
774.75 KB
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MFCS3b.DAT
787.87 KB
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MFCS4a.DAT
783.42 KB
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MFCS4b.DAT
794.06 KB
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MFCS5a.DAT
786.45 KB
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MFCS5b.DAT
759.67 KB
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MFCS6a.DAT
795.82 KB
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MFCS6b.DAT
792.89 KB
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MFCS7a.DAT
785.54 KB
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MFCS7b.DAT
783.97 KB
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MFCS9a.DAT
800.70 KB
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MFCS9b.DAT
810.16 KB
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READ ME for Aging.doc
80.38 KB
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RRSlide10b.Cy3.TIF
6.12 MB
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RRSlide10b.Cy5.TIF
6.12 MB
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RRSlide11b.Cy3.TIF
6.01 MB
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RRSlide11b.Cy5.TIF
6.01 MB
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RRSlide16a.Cy3.Tif
5.91 MB
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RRSlide16a.Cy5.Tif
5.91 MB
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RRSlide16b.Cy3.Tif
5.93 MB
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RRSlide16b.Cy5.Tif
5.93 MB
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RRslide2a.DAT
813.15 KB
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RRslide2b.DAT
883.18 KB
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SexSpecificList.txt
37.42 KB
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SGAsig.xls
167.42 KB
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Template.pdf
1.68 MB
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UMFOS.txt
8.15 MB
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UMFOS.xls
10.70 MB
Abstract
Here we present a statistically rigorous approach to quantifying microarray expression data that allows the relative effects of multiple classes of treatment to be compared and incorporates analytical methods that are common to quantitative genetics. From the magnitude of gene effects and contributions of variance components, we find that gene expression in adult flies is affected most strongly by sex, less so by genotype and only weakly by age (for 1- and 6-wk flies); in addition, sex x genotype interactions may be present for as much as 10% of the Drosophila transcriptome. This interpretation is compromised to some extent by statistical issues relating to power and experimental design. Nevertheless, we show that changes in expression as small as 1.2-fold can be highly significant. Genotypic contributions to transcriptional variance may be of a similar magnitude to those relating to some quantitative phenotypes and should be considered when assessing the significance of experimental treatments.