Data from: Biodiversity assessment using next-generation sequencing: comparison of phylogenetic and functional diversity between Nebraska grasslands
Ahrendsen, Dakota L., University of Nebraska at Omaha
Aust, Shelly K., University of Nebraska at Omaha
Kellar, P. Roxanne, University of Nebraska at Omaha
Published Aug 12, 2016 on Dryad.
Cite this dataset
Ahrendsen, Dakota L.; Aust, Shelly K.; Kellar, P. Roxanne (2016). Data from: Biodiversity assessment using next-generation sequencing: comparison of phylogenetic and functional diversity between Nebraska grasslands [Dataset]. Dryad. https://doi.org/10.5061/dryad.125p4
Global biodiversity is declining rapidly as a consequence of anthropogenic changes to the environment. Traditional diversity indices such as species richness have been used to assess biodiversity, but recent arguments call for a more comprehensive assessment that includes both phylogenetic and functional diversity (PD and FD, respectively). Many PD metrics have been developed, but few empirical studies have compared metrics across sites with the goal of understanding their application to characterizing biodiversity. In this study, 17 PD metrics, four traditional diversity indices, and one measure of FD were calculated and compared between two Nebraska grasslands. PD metrics were calculated from robust phylogenies estimated from next-generation sequencing data of 45 species. Traditional indices were calculated using species abundance data, and FD was quantified by measuring the phylogenetic signal, K, of specific leaf area (SLA). Results showed that PD metrics and traditional indices were not always correlated, and various PD metrics characterized biodiversity differently. In addition, phylogenies estimated from >80 genes were more robust than single- or dual-gene phylogenies resulting in more reliable PD metrics. K of SLA indicated random trait assembly in all sites. Results suggested that metrics that identify phylogenetic structure and relatedness can provide information to conservation planners about the ability of a community to persist in an unpredictable future. A combination of these results with those of future investigations applying PD and FD metrics to varying communities will support concrete recommendations to conservation planners about how to incorporate these metrics into the selection of priority regions.
Sequence alignment of 73 plastid genes, nine mitochondrial genes, and three nuclear repeat regions for 42 rosid taxa plus 3 outgroups
Sequence alignment of the plastid matK gene for 42 rosid taxa plus 3 outgroups
Sequence alignment of the plastid rbcL gene for 42 rosid taxa plus 3 outgroups
Sequence alignment of concatenated plastid matK and rbcL genes for 42 rosid taxa plus 3 outgroups