Data from: Flow imaging microscopy as a novel tool for high-throughput evaluation of elastin-like polymer coacervates
Morales, Marissa A.
Halpern, Jeffrey Mark
Balog, Eva Rose M.
Published Jul 19, 2019 on Dryad.
Cite this dataset
Marvin, Laura et al. (2019). Data from: Flow imaging microscopy as a novel tool for high-throughput evaluation of elastin-like polymer coacervates [Dataset]. Dryad. https://doi.org/10.5061/dryad.14hq8cd
Biological and bioinspired polymer microparticles have broad biomedical and industrial applications, including drug delivery, tissue engineering, surface modification, environmental remediation, imaging, and sensing. Full realization of the potential of biopolymer microparticles will require methods for rigorous characterization of particle sizes, morphologies, and dynamics, so that researchers may correlate particle characteristics with synthesis methods and desired functions. Toward this end, we evaluated biopolymer microparticles using flow imaging microscopy. This technology is widely used in the biopharmaceutical industry but is not yet well-known among the materials community. Our polymer, a genetically engineered elastin-like polypeptide (ELP), self-assembles into micron-scale coacervates. We performed flow imaging of ELP coacervates using two different instruments, one with a lower size limit of approximately 2 microns, the other with a lower size limit of approximately 300 nanometers. We validated flow imaging results by comparison with dynamic light scattering and atomic force microscopy analyses. We explored the effects of various solvent conditions on ELP coacervate size, morphology, and behavior, such as the dispersion of single particles versus aggregates. We found that flow imaging is a superior tool for rapid and thorough particle analysis of ELP coacervates in solution. We anticipate that researchers studying many types of microscale protein or polymer assemblies will be interested in flow imaging as a tool for quantitative, solution-based characterization.
I40 ELP DNA sequencing data
The files in this folder are the sequencing results received from Eurofins Genomics for the I40 POE-W plasmid.
I40 ELP DLS data and analysis
The files in this folder were used to generate Fig 2.
I40 ELP AFM data
IGOR Pro binary wave file containing AFM data used to generate Fig 3.
I40 ELP FlowCam filters and classification files
I40 ELP data analysis and graphics - Origin
Unicode-compliant Origin 2018 project file containing all data analysis and graphing for this study
I40 ELP FlowCam in water
I40 ELP FlowCam in high salt non-reducing conditions
I40 ELP FlowCam in high salt reducing conditions
I40 ELP FlowCam in water - Nano Run 2
I40 ELP FlowCam in water - Nano Run 3
I40 ELP FlowCam in water - Nano Run 4
Description of all directories and files contained in this data package.