Data from: Molecular phylogenetics of Braconidae (Hymenoptera: Ichneumonoidea) based on multiple nuclear genes and implications for classification
Sharanowski, Barbara J., University of Manitoba
Dowling, Ashley P.G., University of Arkansas System
Sharkey, Michael J., University of Kentucky
Published May 16, 2011 on Dryad.
Cite this dataset
Sharanowski, Barbara J.; Dowling, Ashley P.G.; Sharkey, Michael J. (2011). Data from: Molecular phylogenetics of Braconidae (Hymenoptera: Ichneumonoidea) based on multiple nuclear genes and implications for classification [Dataset]. Dryad. https://doi.org/10.5061/dryad.1688p
This study examined subfamilial relationships within Braconidae, using 4kb of sequence data for 139 taxa. Genetic sampling included previously used markers for phylogenetic studies of Braconidae (28S and 18S rDNA) as well as new nuclear protein-coding genes (CAD and ACC). Maximum likelihood and Bayesian inference of the concatenated dataset recovered a robust phylogeny, particularly for early divergences within the family. This study focused primarily on non-cyclostome subfamilies, but the monophyly of the cyclostome complex was strongly supported. There was evidence supporting an independent clade, termed the aphidioid complex as sister to the cyclostome complex of subfamilies. Maxfischeria was removed from Helconinae and placed within its own subfamily within the aphidioid complex. Most relationships within the cyclostome complex were poorly supported, likely due to lower taxonomic sampling within this group. Similar to other studies, there was strong support for the alysioid subcomplex containing Gnamptodontinae, Alysiinae, Opiinae, and Exothecinae. Cenocoeliinae was recovered as sister to all other subfamilies within the euphoroid complex. Planitorus and Mannokeraia, previously placed in Betylobraconinae and Masoninae, respectively, were moved to the Euphorinae and may share a close affiliation with Neoneurinae. Neoneurinae and Ecnomiinae were placed as tribes within Euphorinae. A sister relationship between the microgastroid and sigalphoid complexes was also recovered. Within the helconoid complex was a well-supported lineage parasitic on lepidopteran larvae (macrocentroid subcomplex). Helconini was raised to subfamily status and was recovered as sister to the macrocentroid subcomplex. Blacinae was demoted to tribal status and placed within the newly circumscribed subfamily Brachistinae, which also contains the tribes Diospilini, Brulleiini, and Brachistini, all formerly in Helconinae.
Plain Nexus file of all genes
Each gene or gene fragment is separated in its own block; protein-coding genes treated with Gblocks; rDNA aligned via secondary structure and with ambiguous regions excluded if the standard deviation of length in that region is > 1
All genes with commands for MrBayes. This partitioning strategy was deemed the best using Bayes factors. The dataset has 8 partitions and is referred to as strategy E in the manuscript. Each gene or gene fragment is separated in its own block; protein-coding genes treated with Gblocks; rDNA aligned via secondary structure and with ambiguous regions excluded if the standard deviation of length in that region is > 1.
Statistics file from Bayes-strategyE
Statistics file from Bayes run for all genes. See also "allgenes_8_partitions_strategy_E" nexus file with associated commands for MrBayes.