Environmental DNA metabarcoding reveals and unpacks a biodiversity conservation paradox in Mediterranean marine reserves
Boulanger, Emilie et al. (2021), Environmental DNA metabarcoding reveals and unpacks a biodiversity conservation paradox in Mediterranean marine reserves, Dryad, Dataset, https://doi.org/10.5061/dryad.18931zcx1
30 L of seawater were collected along 2 km transects and filtered using 0.20-µM filtration capsules. Environmental DNA was extracted and amplified by PCR with the fish-specific primer pair teleo targeting a 70 bp fragment at the end of the mitochondrial DNA 12S rRNA gene. PCR reactions were carried out in 12 replicates per sample and unique tags were given to each sample. A paired-end sequencing (2x125 bp) was carried out on a MiSeq (Illumina, San Diego, CA, USA).
Compressed file containing the compressed forward and reverse sequence files of runs 1 through 4.
File linking the sequence files to the sample information.
column 1: run code
column 2: run type
column 3: project name
column 4: sample name (lab identifier, starts with "SPY")
column 5: sample name with PCR replicate number
column 6: tag name = position on plate
column 7: tag sequence
column 8: forward primer sequence
column 9: reverse primer sequence
File linking the sample laboratory ID to the sample (field) metadata.
Assigning the field ID allows to run the analyses on https://github.com/eboulanger/MEDeDNA--reserves
column 1: sample lab code
column 2: sample field code
column 3: sampling date
column 4: longitude coordinate of the transect center
column 5: latitude coordinate of the transect center
New mediterranean fish reference sequences for the teleo barcode.
column 1: local reference database identifier
column 2: lab code
column 3: field code
column 4: species scientific name
column 5: teleo sequence
Agence de l'Eau Rhône Méditerranée Corse