Relict from the Jurassic: New family of brittle-stars from a New Caledonian seamount
Data files
Apr 02, 2021 version files 4.99 GB
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MV_Ophiojura_IE-2007-6821_S01_yz1.zip
2.26 GB
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MV_Ophiojura_IE-2007-6821_S01.zip
2.48 GB
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Ophiojura_195taxa_353gene_ASTRAL_speciestree.tre
10.45 KB
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Ophiojura_195taxa_353genetrees.phy
5.16 MB
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Ophiojura_195taxa_RAxML_bipartitions.nwk
15.95 KB
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Ophiojura_195taxa.phy
51.73 MB
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Ophiojura_79taxa_BEAST_MCCtree.tre
88.13 KB
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Ophiojura_79taxa.xml
3.69 MB
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Ophiojura_CT_Files_readme.txt
1.24 KB
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Ophiojura_rendered_CT_images.zip
182.55 MB
Abstract
Methods
Micro-CT scanning. Performed at the Melbourne TrACEES (Trace Analysis for Chemical, Earth and Environmental Sciences) Platform by Dr. Jay Black (University of Melbourne) using a Phoenix Nanotom m operated using xs control and Phoenix datos|x acquisition software (Waygate Technologies). The specimen was mounted by wrapping in ethanol soak gauze, secured by bubble wrap, and placed within a plastic specimen jar. An x-ray energy of 50kV and 300 mA was used for two separate scans, one at a coarser voxel resolution of 25 micrometre to capture the full body of the specimen and a higher resolution scan at 10 micrometre focusing on a region of interest encompassing the main body. Scans were run for 10 minutes in a fast scan mode collecting 1200 projections through a 360 degree rotation of the specimen. Volume reconstruction of the micro-CT data was performed using Phoenix datos|x reconstruction software (Waygate Technologies) applying an inline median filter and ROI filter during reconstruction. The data was exported as 16-bit volume files for imaging and analysis in Avizo (Thermo Fisher Scientific).
Phylogenetic analyses. Bayesian dating estimates were obtained using BEAST2 v2.4.7 using a codon position GTR+G+F sequence evolution model, with lognormal relaxed-clock model and Yule tree prior (both using 1/X parameterization). Fossil calibrations are outlined in electronic supplementary material Table S3. Maximum likelihood estimates were were generated in RAxML v8.1.20 using a codon position GTR+G+F sequence evolution model by running 200 non-parametric bootstraps (the –f i command) that retain branch lengths per bootstrap. A species tree was estimated using ASTRAL-II, with local posterior support values [19], from 353 (the most data rich, electronic supplementary material, table S2) separate gene trees, drawn from the full 265kb 195 taxa dataset. We used genes (rather than exons) as independent loci [20]. Unrooted gene trees were generated by RAxML (-f d command) using a single partition GTR+G+F model (due to reduced information per gene as compared to the full concatenated data). These gene datasets comprised (median) 189 taxa and 513 sites, with each of the 195 taxa having (median) 343 gene trees (330 for specimen IE.2007.6821). The ASTRAL species tree figure is presented as rooted for ease of comparison.
Usage notes
This dataset includes
a) MV_Ophiojura_IE-2007-6821_S01.zip contains a tiff stack of CT-scan images taken vertically through the holotype of Ophiojura exbodi
b) MV_Ophiojura_IE-2007-6821_S01_yz.zip contains a tiff stack of CT-scan images taken horizontally through the holotype of Ophiojura exbodi)
c) Ophiojura rendered CT images.zip contains various rendered CT-scan images of Ophiojura exbodi showing ventral and dorsal sides of the animal, in black and white and false colour, and an animation of the animal rotating around a horizontal axis.
d) Ophiojura_79taxa.xml is the BEAST XML input file that was used to create the BEAST phylogenic tree in the paper.
e) Ophiojura_79taxa_BEAST MCCtree.tre is the phylogenetic tree that resulted from the BEAST analysis (published as electronic supplement Figure S2b)
f) Ophiojura_195taxa.phy is the input file for the RAxML phylogenetic analysis.
g) Ophiojura_195taxa_RAxML_bipartitions.nwk contains the trees resulting from the RAxML analysis (published as electronic supplement Figure S1a)
h) Ophiojura_195taxa_353genetrees.phy is the input file for the ASTRAL-II analysis
i) Ophiojura_195taxa_353gene_ASTRAL_speciestree.tre contains the ASTRAL trees (published as electronic supplement Figure S1b)