Zona incerta distributes a broad movement signal that modulates behavior
Cite this dataset
Castro-Alamancos, Manuel A. et al. (2023). Zona incerta distributes a broad movement signal that modulates behavior [Dataset]. Dryad. https://doi.org/10.5061/dryad.18931zd42
Abstract
The zona incerta is a subthalamic nucleus made up mostly of GABAergic neurons. It has wide- ranging inputs and outputs and is believed to have many integrative functions that link sensory stimuli with motor responses to guide behavior. However, its role is not well established perhaps because few studies have measured the activity of zona incerta neurons in behaving animals under different conditions. To record the activity of zona incerta neurons during exploratory and cue-driven goal-directed behaviors, we used electrophysiology in head-fixed mice moving on a spherical treadmill and fiber photometry in freely moving mice. We found two groups of neurons based on their sensitivity to movement, with a minority of neurons responding to whisker stimuli. Furthermore, zona incerta GABAergic neurons robustly code the occurrence of exploratory and goal-directed movements, but not their direction. To understand the function of these activations, we performed genetically targeted lesions and optogenetic manipulations of zona incerta GABAergic neurons during exploratory and goal-directed behaviors. The results showed that the zona incerta has a role in modulating the movement associated with these behaviors, but this has little impact on overall performance. Zona incerta neurons distribute a broad corollary signal of movement occurrence to their diverse projection sites, which regulates behavior.
README: Zona incerta distributes a broad movement signal that modulates behavior
The data correspond to the figures and panels shown in the published paper.
Description of the data and file structure
All files are .ogwu books from Originlab. Find the free reader online here: https://www.originlab.com/viewer/
All files are labeled according to the figure, plot, and trace/column color they correspond to in the paper.
The books include defining metadata shown as parameter rows or indexed columns. These refer to information related to the plots shown in the paper. Each book contains traces (time series) or measured values as noted below. Any additional data or metadata in the books (not listed below) are not detailed/included in the related paper.
Fig1C:
Event: (=) Cxp or (=) Cxp4
Class2: type of cell
Site: site ID
Fig2AB:
Long Name: stimulation protocol
Cell: cell ID
Class1: type of cell
Class2: type of cell
Fig3BC:
File: filename
Event: event ID
Variable: variable ID (as explained in the paper)
Class1: type of cell
Animal: animal ID
EventVar: variable + event IDs
Site: site ID
Fig4-6 and Fig8CD:
Traces:
File: filename
Animal: animal ID
SesName: name of session
Session: session number
getFile: animal ID + traced variable ID
Measures:
File: filename
Variable: measured variable ID
Animal: animal ID
Area: measured area under the curve
Max: measured max value
xMax: time to Max
Min: measured min value
xMin: time to Min
Pk: measured absolute peak value
xPk: time to Pk
Fig8B:
Animal: animal ID
Session: session number
Code: session code
NumofTrial: number of total trials in session
Avoids%: percentage of avoids in session
Avoid Latency: latency of avoid responses
ITCs/trial: number of intertrial crossings per trial
Fig9-11:
Traces:
Animal: animal ID
Session: session number
SubSes: subsession ID
NewCode1: light stimulation
Subgroup: animal group ID (e.g. No Opsin, Vgat-ZI-Arch)
Measures:
Subgroup: animal group ID
Animal: animal ID
Session: session number
SubSes: subsession ID
NewCode1: light stimulation
Pk7: measured absolute peak value (0-4 secs)
Sharing/Access information
None.
Code/Software
No code is included. The raw data allows users to obtain the measures with their preferred method.
Methods
The details are described in the associated eLife paper.
Funding
National Institute of Neurological Disorders and Stroke