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Zona incerta distributes a broad movement signal that modulates behavior

Cite this dataset

Castro-Alamancos, Manuel A. et al. (2023). Zona incerta distributes a broad movement signal that modulates behavior [Dataset]. Dryad. https://doi.org/10.5061/dryad.18931zd42

Abstract

The zona incerta is a subthalamic nucleus made up mostly of GABAergic neurons. It has wide- ranging inputs and outputs and is believed to have many integrative functions that link sensory stimuli with motor responses to guide behavior. However, its role is not well established perhaps because few studies have measured the activity of zona incerta neurons in behaving animals under different conditions. To record the activity of zona incerta neurons during exploratory and cue-driven goal-directed behaviors, we used electrophysiology in head-fixed mice moving on a spherical treadmill and fiber photometry in freely moving mice. We found two groups of neurons based on their sensitivity to movement, with a minority of neurons responding to whisker stimuli. Furthermore, zona incerta GABAergic neurons robustly code the occurrence of exploratory and goal-directed movements, but not their direction. To understand the function of these activations, we performed genetically targeted lesions and optogenetic manipulations of zona incerta GABAergic neurons during exploratory and goal-directed behaviors. The results showed that the zona incerta has a role in modulating the movement associated with these behaviors, but this has little impact on overall performance. Zona incerta neurons distribute a broad corollary signal of movement occurrence to their diverse projection sites, which regulates behavior.

README: Zona incerta distributes a broad movement signal that modulates behavior

The data correspond to the figures and panels shown in the published paper.

Description of the data and file structure

All files are .ogwu books from Originlab. Find the free reader online here: https://www.originlab.com/viewer/

All files are labeled according to the figure, plot, and trace/column color they correspond to in the paper.

The books include defining metadata shown as parameter rows or indexed columns. These refer to information related to the plots shown in the paper. Each book contains traces (time series) or measured values as noted below. Any additional data or metadata in the books (not listed below) are not detailed/included in the related paper.

Fig1C:

Event: (=) Cxp or (=) Cxp4

Class2: type of cell

Site: site ID

Fig2AB:

Long Name: stimulation protocol

Cell: cell ID

Class1: type of cell

Class2: type of cell

Fig3BC:

File: filename

Event: event ID

Variable: variable ID (as explained in the paper)

Class1: type of cell

Animal: animal ID

EventVar: variable + event IDs

Site: site ID

Fig4-6 and Fig8CD:

Traces:

File: filename

Animal: animal ID

SesName: name of session

Session: session number

 getFile: animal ID + traced variable ID

Measures:

 File: filename

Variable: measured variable ID

Animal: animal ID

Area: measured area under the curve

Max: measured max value

xMax: time to Max

Min: measured min value

xMin: time to Min

Pk: measured absolute peak value

xPk: time to Pk

Fig8B:

Animal: animal ID

Session: session number

Code: session code

NumofTrial: number of total trials in session

Avoids%: percentage of avoids in session

Avoid Latency: latency of avoid responses

ITCs/trial: number of intertrial crossings per trial

Fig9-11:

Traces:

Animal: animal ID

Session: session number

SubSes: subsession ID

NewCode1: light stimulation

Subgroup: animal group ID (e.g. No Opsin, Vgat-ZI-Arch)

Measures:

 Subgroup: animal group ID

Animal: animal ID

Session: session number

SubSes: subsession ID

NewCode1: light stimulation

Pk7: measured absolute peak value (0-4 secs)

Sharing/Access information

None.

Code/Software

No code is included. The raw data allows users to obtain the measures with their preferred method.

Methods

The details are described in the associated eLife paper. 

Funding

National Institute of Neurological Disorders and Stroke