Strength in numbers? Cytotype frequency mediates effect of reproductive barriers in mixed-ploidy arrays.
Cite this dataset
Sutherland, Brittany; Miranda-Katz, Tomas; Galloway, Laura (2020). Strength in numbers? Cytotype frequency mediates effect of reproductive barriers in mixed-ploidy arrays. [Dataset]. Dryad. https://doi.org/10.5061/dryad.1c59zw3sq
Abstract
Methods
Pollinator visitation observations were made visually and were collected by hand on paper by T. Miranda-Katz and B. Sutherland. Data were then transcribed into a spreadsheet by B. Sutherland. Visitations in which only one plant were visited were then removed from the dataset to be analyzed. Both raw bout information and processed data for each bout (ratios of intra- and intercytotype visitations) are reported.
Fruit set, Seed set, and Germination data were assessed visually and transcribed into a spreadsheet.
F1 cytometric data was collected using a BD FACSCalibur flow cytometer. Modal value for fluorescence for each sample was recorded and compared to either an internal or external standard. Modal value/standard ratios were then compared to the same ratio for known diploid plants to determine ploidy. F1 ploidy, maternal ploidy, ploidy pair, and maternal frequency are recorded.
Usage notes
Missing values are recorded as "."
Tabs containing encoded information (i.e. weather type, insect type, population, etc) is included in each Excel file as relevant.
Funding
National Science Foundation, Award: DEB-1457686
National Science Foundation, Award: DBI-1461169