Data and code from: Soil decomposer can regulate the legacy effect of photodegradation on forest marcescent litter decomposition, but emerging microplastics disrupt this
Data files
Jan 22, 2025 version files 26.29 KB
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README.md
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XinData.zip
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Abstract
Photodegradation—photochemical mineralization of standing litters—often exerts a legacy effect aiding biodegradation in soil (PLE), which is overlooked in deciduous forest containing marcescent leaves. Meanwhile, increasing anthropogenic microplastics have deposited in forests, how they would affect the PLE on subsequent litter bio-decomposition is currently unknown. We employed ultraviolet accelerated ageing chamber to replicate the abiotic photodegradation process (AP) of a naturally marcescent tree Lindera glauca, then manipulated mesocosm bio-incubations to quantify how decomposer (microbial alone or with soil animals) and microplastic contamination would interactively affect the PLE. We found AP significantly decreased litter lignin content before and after the bio-incubation. During an early phase decomposition, lignin lost greatly and displayed a crucial role in determining the ways that soil animal and photodegradation affect the bio-decomposition. Microbial decomposer alone led to a positive PLE universally. Soil animals depressed microbial biomass and inhibited the microbial-mediated PLE in unpolluted mesocosms, but intensified the PLE in contaminated soils. We conclude that decomposer interactions can attenuate PLE, but microplastics will disrupt the established equilibrium, making contaminated soil more susceptible to photodegradation-induced litter chemical changes. This arouses integration of radiation and emerging pollution to further our understanding of biogeochemical cycle in forest.
README: Data and code from：Soil decomposer can regulate the legacy effect of photodegradation on forest marcescent litter decomposition, but emerging microplastics disrupt this
https://doi.org/10.5061/dryad.1g1jwsv4m
Contact Xin Wang (wangxin_unique@163.com ) or Kai Tian (tiankai444422@163.com ) with any questions.
Data files include "XinData.zip" and "R code":
This compressed file contains all the data and code for this manuscript. All data were collected by the authors.
The “XinData.zip” file name and description are as follows:
### litterquality.txt = data on litter mass loss, lignin loss,Closs,Nloss,CNratio and ligninNratio:
mass loss: mass loss rata of litter (unit: %) after 90 days of decomposition;
lignin loss: lignin loss rata of litter (unit: %) after 90 days of decomposition;
Closs: carbon loss rata of litter (unit: %) after 90 days of decomposition;
Nloss: nitrogen loss rata of litter (unit: %) after 90 days of decomposition;
CNratio: the ratio of carbon content to nitrogen content in litter;
ligninNratio: the ratio of lignin content to nitrogen content in litter.
In addation,
The first letters in the first columns of the table stand for,
P+H-A- :with photodegradation;
P-H-A- :non-photodegradation;
P+H+A-: photodegradation and HDPE;
P-H+A-: non-photodegradation with HDPE;
P+H-A+: photodegradation and soil animals;
P-H-A+: non-photodegradation with soil animals;
P+H+A+: photodegradation with HDPE and Soil animals;
P-H+A+: non-photodegradation with HDPE and soil animals;
The first letters in the last three columns of the table stand for,
P: litter with (1) or without (2) photodegradation;
H: with (1) or without (2) High density polyethylene microplastics (HDPE-MPs);
A: with (1) or without (2) soil animals.
### earthworm.txt = data on earthworm weight growth rate:
Treatment: with HDPE-MPs absent (Unpolluted) or present (H. Polluted);
weightloss: earthworm weight growth rate (%)
### Shannon.txt = data on litter fungi shannon index:
treatment: soil with HDPE-MPs absent (Unpolluted) or present (H. Polluted);
shannonentropy: litter fungi shannon index.
### MBCN.csv = data on soil MBC and MBN:
Empty cells: not available (missing value).
treatment: A(soil animals), NoA(without soil animals), A.H (soil animals & HDPE-MPs), NoA.H (without soil animals&withHDPE-MPs);
MBC: Microbial Biomass carbon (mg/kg);
MBN: Microbial Biomass nitrogen (mg/kg);
### PLE.csv = data on PLE (legacy effect of photodegradation) of litter:
Treatment: with (M.A) or without (M) the impact of soil animals;
group:
the PLEs on mass loss (massloss) and lignin loss (ligninloss) in environments without HDPE-MPs containing;
the PLEs on mass loss (massloss.H) and lignin loss (ligninloss.H) in environments with HDPE-MPs containing;
effect.size: effect size of the legacy effect of pre-photodegradation (PLE) on subsequent bio-decomposition.
### A.PLE.txt = data on Effects of soil animals on PLE:
A.PLE : Soil animal's regulatory influences on PLE (A.PLE) of lignin loss and mass loss;
group:
the A.PLEs on mass loss (massloss) and lignin loss (ligninloss) in environments without HDPE-MPs containing;
the A.PLEs on mass loss (massloss.H) and lignin loss (ligninloss.H) in environments with HDPE-MPs containing.
### unpolluted.xlsx = data on structural equation model in unpolluted environment:
Teatment:
P+H-A+: photodegradation and soil animals;
P-H-A+: non-photodegradation with soil animals;
P+H-A- :with photodegradation;
P-H-A- :non-photodegradation;
Soilfauna: with (1) or without (0) soil animals;
UV:litter with (1) or without (0) photodegradation;
CBH1: cellobiohydrolase (μmol·h-1·g-1);
BG: β-1,4-glucosidase (μmol·h-1·g-1);
massloss: mass loss rata of litter (unit: %) after 90 days of decomposition;
UVB:
Initial lignin nitrogen ratio of litter with photodegradation (2.44);
Initial lignin nitrogen ratio of litter without photodegradation (3.47).
ligninloss1:lignin lossof litter after 90 days of decomposition.
###H-polluted = data on structural equation model in H.Polluted environment
treatment:
P+H+A-: photodegradation and HDPE;
P-H+A-: non-photodegradation with HDPE;
P+H+A+: photodegradation with HDPE and Soil animals;
P-H+A+: non-photodegradation with HDPE and soil animals;
Soilfauna: with (1) or without (0) soil animals;
UV:litter with (1) or without (0) photodegradation;
CBH1: cellobiohydrolase (μmol·h-1·g-1);
BG: β-1,4-glucosidase (μmol·h-1·g-1);
massloss: mass loss rata of litter (unit: %) after 90 days of decomposition;
UVB:
Initial lignin nitrogen ratio of litter with photodegradation (2.44);
Initial lignin nitrogen ratio of litter without photodegradation (3.47).
ligninloss1:lignin lossof litter after 90 days of decomposition.
### pca.csv = data on soil extracellular enzyme activity:
Photo: litter with (1) or without (2) photodegradation;
MPs: with (1) or without (2) High density polyethylene microplastics (HDPE-MPs);
fauna: with (1) or without (2) soil animals.
Pheno: Soil phenol oxidase activity (μmol·h-1·g-1);
Perox: Soil Phenol peroxidase activity (μmol·h-1·g-1);
Ure: Soil urease activity activity (mg·h-1·g-1);
NR: Soil nitrate reductase activity (mg·h-1·g-1);
ACP: Soil acid phosphatase activity (mg·h-1·g-1);
CBH1: Soilcellobiohydrolase activity (μmol·h-1·g-1);
BG: Soil β-1,4-glucosidase activity (μmol·h-1·g-1);
BX: Soil β-1,4-xylosidase activity (μmol·h-1·g-1);
Code/Software：
# code.R = R code for the data analyses
This script has been run correctly in R version 4.3.2（R Development Core Team, https://www.r-project.org）