Data from: Spatial structure imposes sex-specific costs but does not reduce interlocus sexual conflict
Data files
Aug 30, 2024 version files 58.87 KB
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Dryad_Habitat_fragmentation_and_Sexual_conflict.xlsx
54.43 KB
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README.md
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Abstract
Spatial structure is a common feature of all naturally occurring populations. Theoretically, spatial structuring of a habitat could modulate the intensity of interlocus sexual conflict (ISC) in a population, possibly by modulating intersexual encounter rate. We tested this theory using laboratory populations of Drosophila melanogaster by measuring male-induced decline in female fitness in three-patch habitat systems with two alternative habitat types: structured–interconnected and unstructured. Our results on reproductive and survival costs in females suggested significant costs attributable to male presence (i.e. ISC) and to living on structured habitat. However, there was only weak evidence supporting the theory of modulation of ISC by habitat structuring only. Through a follow-up experiment, we also showed that the effect of habitat on ISC is robust to the alteration of female conditions. Therefore, it appears that spatial structuring per se is unlikely to modulate ISC but can impose additional survival costs. We also showed that this survival cost could be sex specific, possibly reflecting female-biased spontaneous dispersal.
README: Data from: Spatial structure imposes sex-specific costs but does not reduce interlocus sexual conflict
Key to reading the data file:
Data from all the experiments reported in the manuscript titled “Spatial structure imposes sex-specific costs but does not reduce interlocus sexual conflict” can be found in the spreadsheet file.
Data of different assays from all the experiments have been separated into different tabs in the spreadsheet. Data are arranged vertically with factors/treatments separated in columns. Columns are clearly labeled. The details of the tabs are following.
Tab - Ex1_Progeny count (Tab 1): Data on progeny output (MPO/Mean Progeny Output) under two types of Male exposures – limited exposure (LE), continuous exposure (CE), and two levels of Habitat i.e., Structured and Unstructured is from Experiment 1(Ex 1). ‘BLOCK’ refers to three randomized blocks mentioned as B_2, B_3, B_5. Count data is unit less.
Tab - Ex1_Starvation survival time (Tab 2): Data on starvation survival time (MST/ Mean Starvation survival time) under two types of Male exposures – limited exposure (LE), continuous exposure (CE) and two levels of Habitat i.e., Structured and Unstructured is from Experiment 1(Ex 1). ‘BLOCK’ refers to three randomized block mentioned as B_2, B_3, B_5. Unit of starvation survival time measure is in hours
Tab - Dietary treatment and dispersal (Tab 3): ‘Treatment’ refers to the levels of dietary manipulation (Control, 20%, 40% and 60%). ‘Sex’ refers to Male (M) and Female (F). ‘BLOCK’ refers to three randomized block mentioned as B_4, B_2, B_3. ‘Trial’ refers to total number of one particular sex i.e., 15 and ‘Dispersal’ refers to the absolute number of flies that dispersed.
Tab - Ex2_Progeny count (Tab 4): Data on progeny output (MPO/Mean Progeny Output) for two types of ‘Female’- Poor (i.e., larval development in 40% diluted food) and Standard under two types of Male exposures – limited exposure (LE), continuous exposure (CE) and two levels of Habitat i.e., Structured and Unstructured is from Experiment 2(Ex 2). ‘BLOCK’ refers to three randomized block mentioned as BL_1, BL_2, BL_3. Count data is unit less.
Tab - Ex2_Starvation survival time (Tab 5): Data on starvation survival time (MST/ Mean Starvation survival time) for two types of ‘Female’- Poor and Standard under two types of under two types of Male exposures – limited exposure (LE), continuous exposure (CE) and two levels of Habitat i.e., Structured and Unstructured is from Experiment 2(Ex 2). ‘BLOCK’ refers to three randomized block mentioned as BL_1, BL_2, BL_3. Unit of starvation survival time measure is in hours.
Tab - Ex2_MST_Male (Tab 6): Data on starvation survival time (MST/ Mean Starvation survival time) for two types of ‘Female’- Poor and Standard and two levels of Habitat i.e., Structured and Unstructured is from Experiment 2(Ex 2). ‘BLOCK’ refers to three randomized block mentioned as BL_1, BL_2, BL_3. Unit of starvation survival time measure is in hours.
Tab - Distribution in 3 patch system (Tab 7):
Proportion of Female: ‘Time’ refers to each time point (1H, 2H, 3H,5H,6H,12H,18H,24H,30H,36H,42H,48H) counting was done. P1SR, P2SR, P3SR refers to Sex Ratio (SR) from Patch 1, 2 and 3 respectively. Here, sex ratio indicates proportion of females.
Total number of flies:P1O, P2O, P3O refers to observed number of flies in patch 1, 2, 3 respectively. P1E, P2E, P3E refers to expected number of flies in patch 1, 2, 3 respectively.
Number of flies in corridor:No’ refers to absolute count of flies, ‘Sex’ refers to Male and Female.
Tab - Sex specific dispersal (Tab 8): ‘Sex’ refers to Male (M) and Female (F). ‘Time’ refers to each time point i.e., 2H, 4H and 6H when dispersal was counted ‘Trial’ refers to total number of one particular sex present in the source patch and ‘Dispersal’ refers to the absolute number of flies that dispersed.
Tab - Relative Reduction (Tab 9): ‘Female’ refers Poor (i.e., larval development in 40% diluted food) and Standard. ‘LE’ refers to progeny output at limited exposure. ‘CE’ refers to progeny output at continuous exposure. ‘Habitat’ refers to two levels of habitat type- Structured and Unstructured. ‘RR’ refers to Relative Reduction. ‘Block’ refers to three randomized block -1, 2 and 3.
Methods
Two randomized block design experiments were conducted on laboratory adapted populations of Drosophila melanogaster. The details of these populations were reported first in Nandy et al. 2016 (https://doi.org/10.1016/j.anbehav.2016.01.019). Laboratory structured habitat was set up by using interconnected plastic vials (for three-patch setups) or one vial and one bottle (for two-patch setups). All traits reported in this dataset were measured in the laboratory - close to the populations' usual natural holding condition (i.e., the laboratory conditions in which they are usually maintained). For full experimental details, see the pre-print - https://www.biorxiv.org/content/10.1101/2022.10.29.514349v3.