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The plot thickens: Haploid and triploid-like thalli, hybridization, and biased mating type ratios in Letharia

Citation

Ament-Velásquez, Sandra Lorena et al. (2020), The plot thickens: Haploid and triploid-like thalli, hybridization, and biased mating type ratios in Letharia, Dryad, Dataset, https://doi.org/10.5061/dryad.1jwstqjtd

Abstract

The study of the reproductive biology of lichen fungal symbionts has been traditionally challenging due to their complex and symbiotic lifestyles. Against the common belief of haploidy, a recent genomic study found a triploid-like signal in Letharia. Here, we used genomic data from a pure culture and from thalli, together with a PCR survey of the MAT locus, to infer the genome organization and reproduction in Letharia. We found that the read count variation in the four Letharia specimens, including the pure culture derived from a single sexual spore of L. lupina, is consistent with haploidy. By contrast, the L. lupina read counts from a thallus' metagenome are triploid-like. Characterization of the mating-type locus revealed a conserved heterothallic configuration across the genus, along with auxiliary genes that we identified. We found that the mating-type distributions are balanced in North America for L. vulpina and L. lupina, suggesting widespread sexual reproduction, but highly skewed in Europe for L. vulpina, consistent with predominant asexuality. Taken together, we propose that Letharia fungi are heterothallic and typically haploid, and provide evidence that triploid-like individuals are rare hybrids between L. lupina and an unknown Letharia lineage, reconciling classic systematic and genetic studies with recent genomic observations.

Methods

This data submission contains the files necessary to re-create several figures of the paper, in particular those that use sequence data extracted from SPAdes assemblies, or produced by PCR and aligned for phylogenetic analyses (including partition files). It also includes the genome assembly of the pure culture from a L. lupina spore, which is used as a reference for a Snakemake pipeline, and the gene annotation of the mating type locus. No columns were removed from the alignments before analyses.

Usage Notes

See included README file.