Data from: Gene expression under chronic heat stress in populations of the mustard hill coral (Porites astreoides) from different thermal environments
Kenkel, Carly D., The University of Texas at Austin
Meyer, Eli, Oregon State University
Matz, Mikhail V., The University of Texas at Austin
Published May 17, 2013 on Dryad.
Cite this dataset
Kenkel, Carly D.; Meyer, Eli; Matz, Mikhail V. (2013). Data from: Gene expression under chronic heat stress in populations of the mustard hill coral (Porites astreoides) from different thermal environments [Dataset]. Dryad. https://doi.org/10.5061/dryad.1kn38
Recent evidence suggests that corals can acclimatize or adapt to local stress factors through differential regulation of their gene expression. Profiling gene expression in corals from diverse environments can elucidate the physiological processes that may be responsible for maximizing coral fitness in their natural habitat and lead to a better understanding of the coral's capacity to survive the effects of global climate change. In an accompanying paper, we show that Porites astreoides from thermally different reef habitats exhibit distinct physiological responses when exposed to 6 weeks of chronic temperature stress in a common garden experiment. Here, we describe expression profiles obtained from the same corals for a panel of 9 previously reported and 10 novel candidate stress response genes identified in a pilot RNA-Seq experiment. The strongest expression change was observed in a novel candidate gene potentially involved in calcification, SLC26, a member of the solute carrier family 26 anion exchangers, which was down-regulated by 92-fold in bleached corals relative to controls. The most notable signature of divergence between coral populations was constitutive up-regulation of metabolic genes in corals from the warmer inshore location, including the gluconeogenesis enzymes pyruvate carboxylase and phosphoenolpyruvate carboxykinase and the lipid beta-oxidation enzyme acyl-CoA dehydrogenase. Our observations highlight several molecular pathways that were not previously implicated in the coral stress response and suggest that host management of energy budgets might play an adaptive role in holobiont thermotolerance.
Porites astreoides transcriptome
This file contains the transcriptome as a .fasta file and associated annotation tables for isogroups and gene ontology terms.
Raw qPCR data
This file contains raw qPCR data, split by short term candidates and long-term candidates. Technical replicates have been averaged to yield a single Cp value per sample per gene.
qPCR Statistical Analyses
This file contains normalized qPCR data for both the short-term and long-term gene candidates, as described in the paper, in addition to an R script for analyzing and plotting data.
Pilot RNAseq Data
This file contains cleaned SOLiD reads (in colorspace) and a table of read counts mapping to the Porites astreoides transcriptome as described in the paper.