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Apolipoprotein L-1 renal risk variants form active channels at the plasma membrane driving cytotoxicity

Citation

Giovinazzo, Joseph et al. (2020), Apolipoprotein L-1 renal risk variants form active channels at the plasma membrane driving cytotoxicity, Dryad, Dataset, https://doi.org/10.5061/dryad.1ns1rn8r3

Abstract

Recently evolved alleles of Apolipoprotein L-1 (APOL1) provide increased protection against African trypanosome parasites while also significantly increasing the risk of developing kidney disease in humans. APOL1 protects against trypanosome infections by forming ion channels within the parasite, causing lysis. While the correlation to kidney disease is robust, there is little consensus concerning the underlying disease mechanism. We show in human cells that the APOL1 renal risk variants have a population of active channels at the plasma membrane, which results in an influx of both Na+ and Ca2+. We propose a model wherein APOL1 channel activity is the upstream event causing cell death, and that the activate-state, plasma membrane-localized channel represents the ideal drug target to combat APOL1-mediated kidney disease.

Funding

National Institute of General Medical Sciences, Award: R01GM34107

National Science Foundation, Award: IOS-1249166