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Data from: Comparative analysis of ear-hole closure identifies epimorphic regeneration as a discrete trait in mammals

Citation

Gawriluk, Thomas R. et al. (2016), Data from: Comparative analysis of ear-hole closure identifies epimorphic regeneration as a discrete trait in mammals, Dryad, Dataset, https://doi.org/10.5061/dryad.1q7j5

Abstract

Why mammals have poor regenerative ability has remained a long-standing question in biology. In regenerating vertebrates, injury can induce a process known as epimorphic regeneration to replace damaged structures. Using a 4-mm ear punch assay across multiple mammalian species, here we show that several Acomys spp. (spiny mice) and Oryctolagus cuniculus completely regenerate tissue, whereas other rodents including MRL/MpJ ‘healer’ mice heal similar injuries by scarring. We demonstrate ear-hole closure is independent of ear size, and closure rate can be modelled with a cubic function. Cellular and genetic analyses reveal that injury induces blastema formation in Acomys cahirinus. Despite cell cycle re-entry in Mus musculus and A. cahirinus, efficient cell cycle progression and proliferation only occurs in spiny mice. Together, our data unite blastema-mediated regeneration in spiny mice with regeneration in other vertebrates such as salamanders, newts and zebrafish, where all healthy adults regenerate in response to injury.

Usage Notes

Funding

National Science Foundation, Award: IOS-1353713