https://doi.org/10.5061/dryad.1rn8pk12h

Data underlying Figs. 2-4 and Figs. S1-S10.

Description of the data and file structure

Data underlying Figs. 2-4, Fig. S1, Figs. S2b-S6b, and Figs. S7b-S10 are provided in .xlsx file format. Data underlying Figs. S2a and S7a  are provided in .tif file format. Details below:

• Figure 2.xls:  data underlying Fig. 2 in the main text. This file contains three tabs.

  • MassPhotometry Counts Tab:
    • Column A: Mass (kDa)
    • Column B: Counts of Kinesin (K)
    • Column C: Counts of Streptavidin (S)
    • Column D: Counts of K:S 0.4:1
    • Column E: Counts of K:S 0.8:1
    • Column F: Counts of K:S 1.6:1
    • Column G: Counts of K:S 2.2:1
    • Column H: Counts of K:S 3.0:1
    • Column I: Counts of K:S 3.6:1
  • Global Fit (red lines) Tab:  red lines in Fig. 2
    • Columns A and B: Mass (kDa) and Best-fit to Counts of Kinesin (K)
    • Column D and E: Mass (kDa) and Best-fit to  Counts of Streptavidin (S)
    • Column G and H: Mass (kDa) and Best-fit to  Counts of K:S 0.4:1
    • Column J and K: Mass (kDa) and Best-fit to  Counts of K:S 0.8:1
    • Column M and N: Mass (kDa) and Best-fit to  Counts of K:S 1.6:1
    • Column P and Q: Mass (kDa) and Best-fit to  Counts of K:S 2.2:1
    • Column S and T: Mass (kDa) and Best-fit to  Counts of K:S 3.0:1
    • Column V and W: Mass (kDa) and Best-fit to  Counts of K:S 3.6:1
  • Individual Fit (black lines) Tab: black lines in Figure 2, right panel.
    • Columns A and B: Mass (kDa) and Best-fit for peak iii in K:S 0.4:1
    • Columns D, E, and F: Mass (kDa) and Best-fits for peaks iii and iv in K:S 0.8:1
    • Columns H, I, and J: Mass (kDa) and Best-fits for peaks iii and iv in K:S 1.6:1
    • Columns L, M, and N: Mass (kDa) and Best-fits for peaks iv and v in K:S 2.2:1
    • Columns P, Q, and R: Mass (kDa) and Best-fits for peaks iv and v in K:S 3.0:1
    • Columns T, U, and V: Mass (kDa) and Best-fits for peaks iv and v in K:S 3.6:1

• Figure 3.xlsx: data underlying Fig. 3 in the main text. This file contains two tabs.

  • Scatter Data Tab:
    • Column A:  Expected mass (kDa)
    • Column B:  Measured mass, mean value (kDa)
    • Column C:  Measured mass, standard deviation (kDa)
  • Linear Fit Tab:  red line in Fig. 3
    • Column A: Expected mass (kDa)
    • Column B: Best-fit to measured mass (kDa)

• Figure 4.xlsx: data underlying Fig. 4 in the main text. This file contains three tabs.

  • Scatter Data Tab:
    • Column A: Kinesin-streptavidin mixing ratio
    • Column B: Relative abundance of 1-kinesin complex (%)
    • Column C:  Error bar in the relative abundance of 1-kinesin complex (%)
    • Column D: Relative abundance of 2-kinesin complex (%)
    • Column E:  Error bar in the relative abundance of 2-kinesin complex (%)
    • Column F: Relative abundance of 3-kinesin complex (%)
    • Column G:  Error bar in the relative abundance of 3-kinesin complex (%)
    • Column H: Relative abundance of higher-order complex (%)
    • Column I:  Error bar in the relative abundance of higher-order complex (%)
  • BindingModel_DashedLines Tab: dashed lines in Fig. 4
    • Column A: Kinesin-streptavidin mixing ratio
    • Column B:  Relative abundance of 1-kinesin complex (%)
    • Column C:  Relative abundance of 2-kinesin complex (%)
    • Column D:  Relative abundance of 3-kinesin complex (%)
    • Column E:  Relative abundance of 4-kinesin complex (%)
  • BindingModel_BlueLines Tab: blue lines in Fig. 4
    • Column A: Kinesin-streptavidin mixing ratio
    • Column B:  Relative abundance of 1-kinesin complex (%)
    • Column C:  Relative abundance of 2-kinesin complex (%)
    • Column D:  Relative abundance of 3-kinesin complex (%)
    • Column E:  Relative abundance of 4-kinesin complex (%)

• Figure S1.xlsx: data underlying Fig. S1 in the supplement. This file contains two tabs.

  • ScatterData Tab:
    • Column A: Mass (kDa)
    • Column B: Interference intensity (a.u.)
  • LinearFit Tab: black line in Fig. S1
    • Column A: Mass (kDa)
    • Column B: Best-fit to interference intensity (a.u.)

• Figure S2a.tif:  gel file for Fig. S2a in the supplement.

• Figure S2b.xlsx: data underlying Fig. S2 in the supplement. This file contains two tabs.

  • ScatterData Tab:
    • Column A: Kinesin dilution relative to Lane 2
    • Column B: Kinesin-streptavidin mixing ratio
  • LinearFit Tab: black line in Fig. S2b
    • Column A: Kinesin dilution relative to Lane 2
    • Column B: Best-fit to kinesin-streptavidin mixing ratio

• Figure S3a.xlsx: data underlying Fig. S3a in the supplement. This file contains one tab.

  • Counts Tab:
    • Column A: Mass (kDa)
    • Column B: Counts for Measurements 1-3
    • Column C: Counts for Measurements 4-6
    • Column D: Counts for Measurements 7-9
    • Column E: Counts for Measurements 10-12
    • Column F: Mean counts for Measurements 1-12
    • Column G: Standard deviation in the counts of Measurements 1-12

• Figure S3b.xlsx: data underlying Fig. S3b in the supplement. This file contains two tabs.

  • Left panel Tab: data for the left panel
    • Column A: Mass (kDa)
    • Column B: Counts for Sample 1
    • Column C: Counts for Sample 2
    • Column D: Mean counts of Sample 1 and 2
    • Column E: Standard deviation of Sample 1 and 2
  • Right panel Tab: data for the right panel
    • Column A: Mass (kDa)
    • Column B: Normalized counts of Sample 1 (%)
    • Column C: Normalized counts of Sample 2 (%)
    • Column D: Mean of the normalized counts of Sample 1 and Sample 2 (%)
    • Column E: Standard deviation of the normalized counts of Sample 1 and Sample 2 (%)

• Figure S4.xlsx: data underlying Fig. S4 in the supplement. This file contains one tab.

  • MassPhotometry Counts Tab:
    • Column A: Mass (kDa)
    • Column B: Counts of Kinesin (K)
    • Column C: Counts of Streptavidin (S)
    • Column D: Counts of K:S 0.4:1
    • Column E: Counts of K:S 0.8:1
    • Column F: Counts of K:S 1.6:1
    • Column G: Counts of K:S 2.2:1
    • Column H: Counts of K:S 3.0:1
    • Column I: Counts of K:S 3.6:1

• Figure S5.xlsx: data underlying Fig. S5 in the supplement. This file contains one tab.

  • Counts Tab:
    • Column A: Mass (kDa)
    • Column B: Counts of K:S 0.8:1
    • Column C: Counts of K:S 3.0:1

• Figure S6a.xlsx: data underlying Fig. S6a in the supplement. This file contains three tabs.

  • Counts Tab:
    • Column A: Mass (kDa)
    • Column B: Counts of Sample 1
    • Column C: Counts of Sample 2
  • Sample 1 Fits Tab: best-fits to Sample 1 data
    • Column A and B: Mass (kDa) and Best-fit for Peak ii
    • Column D and E: Mass (kDa) and Best-fit for Peak iii
    • Column G and H: Mass (kDa) and Best-fit for Peak iv
    • Column J and K: Mass (kDa) and Best-fit for Peak v
  • Sample 2 Fits Tab: best-fits to Sample 2 data
    • Column A and B: Mass (kDa) and Best-fit for Peak ii
    • Column D and E: Mass (kDa) and Best-fit for Peak iii
    • Column G and H: Mass (kDa) and Best-fit for Peak iv
    • Column J and K: Mass (kDa) and Best-fit for Peak v

• Figure S6b.xlsx: data underlying Fig. S6b in the supplement. This file contains one tab.

  • Column A: Kinesin-streptavidin complex species
  • Column B: Relative abundance of complex species for Sample 1 (%)
  • Column C: Relative abundance of complex species for Sample 2 (%)

• Figure S7a_kinesin.tif: gel file for Fig. S7a (kinesin) in the supplement.

• Figure S7a_streptavidin.tif: gel file for Fig. S7a (streptavidin) in the supplement.

• Figure S7b.xlsx: data underlying Fig. S7b in the supplement. This file contains one tab.

  • Counts Tab:
    • Column A: Deconvoluted mass (Da)
    • Column B: Counts

• Figure S8.xlsx: data underlying Fig. S8 in the supplement. This file contains one tab.

  • Sheet1 Tab:
    • Column A & Rows 2-102: Overall scaling for mixing ratio
    • Column B-AF & Row 1: % kinesin biotinylation
    • Column B-AF & Rows 2-102: Reduced 𝜒2 for each combination of the % kinesin-biotinylation efficiency and the overall scaling for the kinesin-streptavidin mixing ratio

• Figure S9.xlsx: data underlying Fig. S9 in the supplement. This file contains one tab.

  • Sheet1 Tab:
    • Column A: Streptavidin concentration (uM)
    • Column B: Relative abundance of 1-kinesin complex (%)
    • Column C: Error bar in the relative abundance of 1-kinesin complex (%)
    • Column D: Relative abundance of 2-kinesin complex (%)
    • Column E: Error bar in the relative abundance of 2-kinesin complex (%)
    • Column F: Relative abundance of 3-kinesin complex (%)
    • Column G: Error bar in the relative abundance of 3-kinesin complex (%)
    • Column H: Relative abundance of higher-order complex (%)
    • Column I: Error bar in the relative abundance of higher-order complex (%)

• Figure S10.xlsx: data underlying Fig. S10 in the supplement. This file contains five tabs.

  • 100% biotinylation Tab:
    • Column A: kinein-streptavidin mixing ratio
    • Column B: Relative abundance of 1-kinesin complex (%)
    • Column C: Relative abundance of 2-kinesin complex (%)
    • Column D: Relative abundance of 3-kinesin complex (%)
    • Column E: Relative abundance of 4-kinesin complex (%)
  • 80% biotinylation Tab:
    • Column A: kinein-streptavidin mixing ratio
    • Column B: Relative abundance of 1-kinesin complex (%)
    • Column C: Relative abundance of 2-kinesin complex (%)
    • Column D: Relative abundance of 3-kinesin complex (%)
    • Column E: Relative abundance of 4-kinesin complex (%)
  • 70% biotinylation Tab:
    • Column A: kinein-streptavidin mixing ratio
    • Column B: Relative abundance of 1-kinesin complex (%)
    • Column C: Relative abundance of 2-kinesin complex (%)
    • Column D: Relative abundance of 3-kinesin complex (%)
    • Column E: Relative abundance of 4-kinesin complex (%)
  • 60% biotinylation Tab:
    • Column A: kinein-streptavidin mixing ratio
    • Column B: Relative abundance of 1-kinesin complex (%)
    • Column C: Relative abundance of 2-kinesin complex (%)
    • Column D: Relative abundance of 3-kinesin complex (%)
    • Column E: Relative abundance of 4-kinesin complex (%)
  • 50% biotinylation Tab:
    • Column A: kinein-streptavidin mixing ratio
    • Column B: Relative abundance of 1-kinesin complex (%)
    • Column C: Relative abundance of 2-kinesin complex (%)
    • Column D: Relative abundance of 3-kinesin complex (%)
    • Column E: Relative abundance of 4-kinesin complex (%)