Data from: Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation, and loss
Martin, Judy Lisette et al. (2018), Data from: Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation, and loss, Dryad, Dataset, https://doi.org/10.5061/dryad.1v1g1b0
Organ renewal is governed by the dynamics of cell division, differentiation, and loss. To study these dynamics in real time, we present a platform for extended live imaging of the adult Drosophila midgut, a premier genetic model for stem cell-based organs. A window cut into a living animal allows the midgut to be imaged while intact and physiologically functioning. This approach prolongs imaging sessions to 12-16 hours and yields movies that document cell and tissue dynamics at vivid spatiotemporal resolution. Applying a pipeline for movie processing and analysis, we uncover new, intriguing cell behaviors: that mitotic stem cells dynamically re-orient, that daughter cells use slow kinetics of Notch activation to reach a fate-specifying threshold, and that enterocytes extrude via ratcheted constriction of a junctional ring. By enabling real-time study of midgut phenomena that were previously inaccessible, our platform opens a new realm for dynamic understanding of adult organ renewal.