The Earth’s ecosystems are affected by a complex interplay of biotic and abiotic factors. While global temperatures increase, associated changes in the fruiting behaviour of fungi remain unknown. Here we analyse 6.1 million fungal fruit body (mushroom) records and show that the major terrestrial biomes exhibit similarities and differences in fruiting events. We observed one main fruiting peak for most years in all biomes. However, in boreal and temperate biomes years with a second peak were prevalent indicating spring and autumn fruiting. Distinct fruiting peaks are spatially synchronized in boreal and temperate biomes, but less defined and longer in the humid tropics. The timing and duration of fungal fruiting are significantly related to temperature mean and variability. Temperature-dependent aboveground fungal fruiting behaviour, which is arguably also representative of belowground processes, suggests that the observed biome-specific differences in fungal phenology will change in space and time when global temperatures continue to increase.