Population genetic structure of Nephrops norvegicus from the Adriatic Sea inferred using microsatellite loci
Data files
Oct 07, 2024 version files 49.15 KB
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Nephrops_10loci.csv
48.04 KB
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README.md
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Abstract
The Nephrops norvegicus, a prized decapod crustacean species, is found at different depths across the East Atlantic Ocean and Mediterranean Sea. Despite management efforts, the stocks are globally characterized as overexploited. In the present study, the impact of biogeographical boundaries on the phylogeographical and demographic population status was investigated within the Adriatic Sea, addressing important genetic indices for decapod functional conservation management.
README
Population genetic structure of Nephrops norvegicus from the Adriatic Sea inferred using microsatellite loci
https://doi.org/10.5061/dryad.1vhhmgr26
A total of 482 samples were successfully genotyped with 10 microsatellite markers developed for Nephrops and Homarus gammarus (André and Knutsen, 2010; Streiff et al., 2001; Skirnisdottir et al., 2010; Table S1). These were divided into three PCR multiplexes and amplified using the Qiagen multiplex kit and standard primer dyes (FAM, NED, VIC, and PET, Applied Biosystems). Fragments were separated on an ABI3130 automated sequencer with the 500 LIZ dye Size Standard (Applied Biosystems) using the services of Macrogen (Macrogen, Seoul, South Korea). Genotypes were analysed using GeneMapper software v.3.5 (Applied Biosystems).
Description of the data and file structure
This is the input file of the program STRUCTURE for 12 populations of decapod Nephrops norvegicus from the Adriatic Sea. The first row contains the marker names. Each individual is represented by two rows. -9 represents missing data.