Antioxidant cysteine and methionine derivatives show trachea disruption in insects
Data files
Oct 01, 2024 version files 19.71 KB
-
Morimura_et_al_2024_data_2024-09-30.zip
13.35 KB
-
README.md
6.36 KB
Abstract
To prevent the deterioration of the global environment, the reduction of chemical pesticide use and the development of eco-friendly pest control technologies are urgent issues. Our recent study revealed that the production of reactive oxygen species (ROS) by dual oxidase (Duox) plays a pivotal role in stabilizing the tracheal network by intermediating the tyrosine cross-linking of proteins that constitute trachea. Notably, the formation of dityrosine bonds by ROS can be inhibited by the intake of an antioxidant cysteine derivative N-acetyl-L-cysteine (NAC), which can suppress insect respiration. In this study, we screened for the derivatives showing insecticidal activity and tracheal formation inhibition. As a result of investigating the soybean pest bug Riptortus pedestris, cysteine and methionine derivatives showed respiratory formation inhibition and high insecticidal activity. In particular, NAC had a slow-acting insecticidal effect, while L-cysteine methyl ester (L-CME) showed relatively fast-acting insecticidal activity. Furthermore, the insecticidal activity of these derivatives was also detected in Drosophila, mealworms, cockroaches, termites, and plant bugs. Our results suggest that some antioxidant compounds have specific tracheal inhibitory activity in different insect species and they may be used as novel pest control agents upon further characterization.
README: Antioxidant cysteine and methionine derivatives show insecticidal activity by disrupting tracheal formation
https://doi.org/10.5061/dryad.2280gb60h
These datasets include Supplementary information from the research paper "Antioxidant cysteine and methionine derivatives show insecticidal activity by disrupting tracheal formation."
Description of the data and file structure
The datasets are included in a Zip file. The files show the raw data of figures. "n/a" means "not applicable," and "null" means "no data" in the datasets.
1. PLoS_ONE_Antioxidant_Rawdata_Fig1.csv
Mortality of R. pedestris nymphs treated with each of the antioxidants (10 mg/ml, pH 6.8) after 14 days of treatment (mean ± SD, n = 3~5). In each replicate, 10 to 15 insects were reared in a petri dish, and the mortality rate was measured. In each replicate, 10 to 15 insects were reared in a petri dish, and the mortality rate was measured.
2. PLoS_ONE_Antioxidant_Rawdata_Fig3.csv
Mortality of R. pedestris nymphs treated with the antioxidants adjusted to 1 mg/ml, pH 6.8. Mortality (%) was measured 7 days after treatment (mean ± SD, n = 3). In each replicate, 10 to 15 insects were reared in a petri dish and mortality rate was measured. Abbreviations: DW, Distilled water; NAC, N-Acetyl-L-cysteine; L-Cys, L-cysteine; L-CME, L-cysteine methyl ester hydrochloride; L-CEE, L-cysteine ethyl ester hydrochloride; D-Cys, D-cysteine; D-CME, D-cysteine methyl ester hydrochloride; D-PA, D-penicillamine; 2-AET, 2-amino ethanethiol; L-Met, L-methionine; L-MME, L-methionine methyl ester hydrochloride.
3. PLoS_ONE_Antioxidant_Rawdata_Fig4E.csv
The adults of R.pedestris were treated by the antioxidant cysteine and methionine derivatives. The mortalities (%) were calculated after 7 days. The concentration was adjusted to 10 mg / ml.
4. PLoS_ONE_Antioxidant_Rawdata_Fig5.csv
Mortality of diverse insect species when they were fed on the antioxidant cysteine derivatives (10 mg/ml, pH 6.8). The numbers on the graph indicate the number of deaths/treated individuals. Mortality was observed at: 7 days after treatment in the green plant bug A. spinolae and japanese termite R. speratus; 14 days after treatment in the red loach B. lateralis and the mealworm T. molitor; 2 days after treatment in Drosophila. Each treatment was compared with the sterile water (showed as "vs_DW") treatment (ns; no significant, p < 0.05; *, p < 0.01; ***, p < 0.005; Fisher's exact test).
5. PLoS_ONE_Antioxidant_Rawdata_FigS3.csv
The survival rate (%) of R. pedestris nymphs treated with distilled water, ascorbic acid, anthocyanin, and urea. Kaplan-Meier curbs (Fig. 1B Controls) were generated by this sheet survival data. Survival rates and nymphal stages of R. pedestris. Observations started from the 3rd instar nymphs. The total numbers of insects at the starting time (day 0) are shown in brackets after the chemical names.
6. PLoS_ONE_Antioxidant_Rawdata_FigS4_2-AET.csv
The survival rate (%) of R. pedestris nymphs treated with 2-amino ethanethiol. Kaplan-Meier curb (Fig. 1B 2-AET) was generated by this sheet survival data. Survival rates and nymphal stages of R. pedestris. Observations started from the 3rd instar nymphs. The total numbers of insects at the starting time (day 0) are shown in brackets after the chemical names. Abbreviation: 2-AET, 2-amino ethanethiol.
7. PLoS_ONE_Antioxidant_Rawdata_FigS4_D-Cysteine_derivatives.csv
The survival rate (%) of R. pedestris nymphs treated with D-Cysteine derivatives. Kaplan-Meier curbs (Fig. 1B D-Cysteine derivatives) were generated by this sheet survival data. Survival rates and nymphal stages of R. pedestris. Observations started from the 3rd instar nymphs. The total numbers of insects at the starting time (day 0) are shown in brackets after the chemical names. Abbreviations: D-Cys, D-cysteine; D-CME, D-cysteine methyl ester hydrochloride; D-PA, D-penicillamine.
8. PLoS_ONE_Antioxidant_Rawdata_FigS4_L-Cysteine_derivatives.csv
The survival rate (%) of R. pedestris nymphs treated with L-Cysteine derivatives. Kaplan-Meier curbs (Fig. 1B L-Cysteine derivatives) were generated by this sheet survival data. Survival rates and nymphal stages of R. pedestris. Observations started from the 3rd instar nymphs. The total numbers of insects at the starting time (day 0) are shown in brackets after the chemical names. Abbreviations: NAC, N-Acetyl-L-cysteine; L-Cys, L-cysteine; L-CME, L-cysteine methyl ester hydrochloride; L-CEE, L-cysteine ethyl ester hydrochloride.
9. PLoS_ONE_Antioxidant_Rawdata_FigS4_L-Methionine_derivatives.csv
The survival rate (%) of R. pedestris nymphs treated with L-Methionine derivatives. Kaplan-Meier curbs (Fig. 1B L-Methionine derivatives) were generated by this sheet survival data. Survival rates and nymphal stages of R. pedestris. Observations started from the 3rd instar nymphs. The total numbers of insects at the starting time (day 0) are shown in brackets after the chemical names. Abbreviations: L-Met, L-methionine; L-MME, L-methionine methyl ester hydrochloride.
10. PLoS_ONE_Antioxidant_Rawdata_FigS5.csv
Mortality (%) of R. pedestris adults fed the antioxidant cysteine and methionine derivatives. Results of female (F) and male (M) are shown. Mortalities were observed 7 days after treatment (mean ± SD, n = 3). In each replicate, five female and five male individuals were reared in a petri dish, and their mortality was observed. Abbreviations: DW, Distilled water; NAC, N-Acetyl-L-cysteine; L-Cys, L-cysteine; L-CME, L-cysteine methyl ester hydrochloride; L-CEE, L-cysteine ethyl ester hydrochloride; D-Cys, D-cysteine; D-CME, D-cysteine methyl
Sharing/Access information
Links to other publicly accessible locations of the data:
Code/Software
We used the software below.
- Prism 10 (Statistical analysis software): https://www.graphpad.com/features
- ImageJ (Image analyzing software): Schneider CA, Rasband W, Eliceiri KW. NHI Image to ImageJ: 25 years of image analysis. Nat Methods. 2012; 9: 671-675. doi: https://doi.org/10.1038/nmeth.2089 PMID: 22930834