Data from: Among-individual behavioural variation in the ornamental red cherry shrimp, Neocaridina heteropoda
Data files
Feb 22, 2024 version files 27.61 KB
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raw.data.sheet.csv
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README.md
Abstract
Personality variation, defined as among-individual differences in behaviour that are repeatable across time and context, has been reported across animal taxa. From an evolutionary perspective, characterising the amount and structure of this variation is useful since differences among-individuals are the raw material for adaptive behavioural evolution. However, it is becoming apparent that behavioural variation among-individuals also has implications for more applied areas of evolution and ecology – from invasion biology, to ecotoxicology, and selective breeding in captive systems. Here, we investigate the structure of personality variation in the red cherry shrimp, Neocaridina heteropoda, a popular ornamental species that is readily kept and bred under lab conditions and is emerging as a decapod crustacean model across these fields, but for which basic biological, ecological, and behavioural data is limited. Using two assays and a repeated measures approach, we quantify behaviours putatively indicative of shy-bold variation and test for sexual dimorphism and/or size-dependent behaviours (as predicted by some state-dependent models of personality). We find moderate to high behavioural repeatabilities across traits. Although strong individual level correlations across behaviours are consistent with a major personality axis underlying these observed traits, the multivariate structure of personality variation does not fully match a priori expectations of a shy-bold axis. This may reflect our ecological naivety with respect to what really constitutes bolder, more risk prone, behaviour in this species. We find no evidence for sexual dimorphism and only weak support size-dependent behaviour. Our study contributes to the growing literature describing behavioural variation in aquatic invertebrates. Furthermore, it lays a foundation for further studies harnessing the potential of this emerging model system. In particular, this existing behavioural variation could be functionally linked to life-history traits and invasive success, and serve as target of artificial selection or bioassays. It thus holds significant promise in applied research across ecotoxicology, aquaculture, and invasion biology.
README: Among-individual behavioural variation in the ornamental red cherry shrimp, Neocaridina heteropoda
Authors
Rosie A. Rickward, F. Santostefano, Alastair J. Wilson,
Data Collection - Rosie A. Rickward
Code writing - Rosie A. Rickward, Francesca Santostefano, Alastair J. Wilson
data file name - 'raw.data.sheet.csv'
Column headers with explanations:
ID: The identity assigned to each shrimp
date: date the assay took place
time.x: The time of day each trial took place
tracklength: The total difstance travelled (cm) in the OFT trial
wall_dist: The Average distance away from tank walls (cm) in the OFT trial
area: The proportion of the total arena covered (%) in the OFT trial
Trial.x: The type of assay and the trial repeat number
time_arena: Time spent in the trackable part of the arena in the FST trial
tl_arena: Distance travelled while outside shelter and food zones (cm) in the FST trial
frz_arena: The total number of freezes in the arena in the FST trial. Defined as speed dropping <4cm. s-1 for ≥25s
shelter_duration: The total time spent in the shelter (s) in the FST trial
Set.Up: The experimental arena used (tank A vs Tank B)
Order: Reflecting the order of individuals tested between experimental water changes (from 1-6)
Sex.mm: Sex recorded for each shrimp estimated from external morphology, after each trial
estimated.sex1: Assigned single sex determination of a male or female if ≥5/6 trial-specific assessments were in agreement. We assigned sex as ‘unknown’ if this criterion was not met
size: The length of each shrimp (from the tip of the rostrum to the posterior end of the telson to the nearest 0.001mm) recorded at the end of each trial
weight: The masss of each shrimp (to the nearest 0.01g) recorded at the end of each trial
OFT_tot_frz: The total number of freezes in the OFT trial. Defined as speed dropping <4cm. s-1 for ≥25s
food_latency2: Time to first entry of the food zone (s) in the FST trial
Missing data code: NA
Additional information: definition of transformed variables used for the final analyses - variables are transformed within the provided R script based on the raw data above
replicate: Trial repeat number for each individual (from 1-3)
res: Time of day each trial took place after transformation to minutes after midnight
tracklength3: The square root transformation and scaling to standard deviation units of 'tracklength'
area2: Scaling to standard deviation units of 'area'
wall_dist2: The log transformation and scaling to standard deviation units of 'wall_dist'
freez2: The square root transformation, sign reversal and scaling to standard deviation units of 'OFT_tot_frz'
dur_NIS2: Time spent outside the shelter zone (s) in the FST trial (240-'shelter_duration'). This was then square root transformed and scaled to standard deviation units
foodlat2: The log transformation, sign reversal and scaling to standard deviation units of 'food_latency2'
ATL2: The square root transformation and scaling to standard deviation units of 'tl_arena'
AFRZ2: The square root transformation, sign reversal and scaling to standard deviation units of 'frz_arena'
time_arena_sqrt: The square root transformation of 'time_arena'
Methods
Refer to Methods in the associated manuscript.