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Data from: The FlbA-regulated predicted transcription factor rpnR of Aspergillus niger is involved in stress resistance and protein secretion, in Regulators Controlled by the Sporulation Gene flbA of Aspergillus niger (Ph.D. thesis)

Citation

Aerts, David (2019), Data from: The FlbA-regulated predicted transcription factor rpnR of Aspergillus niger is involved in stress resistance and protein secretion, in Regulators Controlled by the Sporulation Gene flbA of Aspergillus niger (Ph.D. thesis), Dryad, Dataset, https://doi.org/10.5061/dryad.25m10

Abstract

Proteins are secreted throughout the mycelium of Aspergillus niger except for the sporulating zone. A link between sporulation and repression of protein secretion was underlined by the finding that inactivation of the sporulation gene flbA results in colonies that secrete proteins throughout the colony. This finding is of interest to improve fungi as a cell factory. However, ΔflbA hyphae also lyse and have thinner cell walls. This pleiotropic phenotype is associated with differential expression of 36 transcription factor genes, of which rpnR was inactivated in this study. Sporulation, biomass, and secretome complexity were not affected in strain ΔrpnR. In contrast, ΔrpnR showed decreased resistance to H2O2 and the proteotoxic stress-inducing agent dithiothreitol. This was associated with reduced ribosomal subunit expression and reduced levels of proteins secreted into the medium. Taken together, RpnR of A. niger is involved in protein synthesis and proteotoxic stress resistance and is thus an interesting target for improving enzyme production capacity.

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