Nexus file containing the sequence alignment for the mitochondrial ND4 haplotypes presented in this study. Sequence labels are field tag identifiers for individual, field-collected specimens. Georeference points for each specimen are available upon request to J. Q. Richmond (jrichmond@usgs.gov). Nucleotide positions 1-1341 encode the NADH4 gene; 1342 - 1411 encodes tHIS; 1412-1476 encodes tSER; and 1477-1486 partially encides tLEU. Gene assignments were based on an alignment with the full mitochondrial genome sequence of Plestiodon egregius.