Data from: Examining the interglacial high-elevation refugia scenario in East Asian subtropical mountain systems with the frog species Leptobrachium liui
Zheng, Yuchi, Chengdu Institute of Biology
Hu, Junhua, Chengdu Institute of Biology
Zeng, Xiaomao, Chengdu Institute of Biology
Published Jul 24, 2019 on Dryad.
Cite this dataset
Zheng, Yuchi; Hu, Junhua; Zeng, Xiaomao (2019). Data from: Examining the interglacial high-elevation refugia scenario in East Asian subtropical mountain systems with the frog species Leptobrachium liui [Dataset]. Dryad. https://doi.org/10.5061/dryad.29kb682
The effects of Quaternary climatic oscillations on the distributions of organisms in different parts of the world are not equally well understood, limiting the ability to understand the determinants of biodiversity. Compared with the mountain regions in southern Europe and southwestern North America, such effects on high-elevation species in the East Asian subtropical mountain systems located in southern and southeastern China have seldom been addressed. In this study, using Leptobrachium liui (Megophryidae), we made one of the earliest attempts to examine the interglacial high-elevation refugia scenario in these Asian mountains. Based on our current understanding of the study system, we formulated a hypothesis that these frogs of western origin were distributed more widely and continuously during glacial phases, allowing eastward dispersal, and that they are currently isolated in interglacial refugia at higher elevations. Microsatellite data and mitochondrial and nuclear sequence data were obtained with extensive sampling followed by the synthesis of phylogeographic and population genetic analyses and modeling of the species distribution. The analyses revealed a sequential eastward divergence of microsatellite clusters and gene lineages accompanied by a decline in genetic diversity. Molecular dating estimates revealed divergence events in the Pleistocene, and a reduction in local populations was inferred to have occurred at a time comparable to the end of the last glacial. Strong genetic isolation by distance reflecting a more continuous historical distribution was detected. Furthermore, environmental niche models inferred a wide planar distribution during the last glacial maximum, providing further support for the hypothesis.
L. liui + L. leishanense, 909 mtDNA sequences
A 745-bp mitochondrial locus containing regions of the tRNA-Leu (16 bp) and NADH dehydrogenase subunit 1 genes; 799 individuals from 22 sampling sites of Leptobrachium liui and 108 individuals from 3 sites of L. leishanense; 2 individuals of L. boringii as the outgroup; alignment in fasta format.
L. liui + L. leishanense, 201 rag1 sequences
An 800-bp partial sequence of the recombinase activating protein 1 gene; 199 sequences from 22 sampling sites of Leptobrachium liui and 3 sites of L. leishanense; 2 sequences of L. ngoclinhense and L. xanthospilum as outgroups; alignment in fasta format.
L. liui + L. leishanense, 832 individuals' STR
832 individuals from 21 populations of Leptobrachium liui and 3 populations of L. leishanense; 8-14 microsatellite loci each population; data in genepop format.