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Morning glory species co-occurrence is associated with asymmetrically decreased and cascading reproductive isolation

Citation

Ostevik, Kate; Rifkin, Joanna; Xia, Hanhan; Rausher, Mark (2020), Morning glory species co-occurrence is associated with asymmetrically decreased and cascading reproductive isolation, Dryad, Dataset, https://doi.org/10.5061/dryad.2bvq83bnx

Abstract

Hybridization between species can affect the strength of the reproductive barriers that separate those species. Two extensions of this effect are: (1) the expectation that asymmetric hybridization or gene flow will have asymmetric effects on reproductive barrier strength and (2) the expectation that local hybridization will affect only local reproductive barrier strength and could therefore alter within-species compatibility. We tested these hypotheses in a pair of morning glory species that exhibit asymmetric gene flow from highly selfing Ipomoea lacunosa into mixed-mating I. cordatotriloba in regions where they co-occur. Because of the direction of this gene flow, we predicted that reproductive barrier strength would be more strongly affected in I. cordatotriloba than I. lacunosa. We also predicted that changes to reproductive barriers in sympatric I. cordatotriloba populations would affect compatibility with allopatric populations of that species.  We tested these predictions by measuring the strength of a reproductive barrier to seed set across the species’ ranges. Consistent with our first prediction, we found that sympatric and allopatric I. lacunosa produce the same number of seeds in crosses with I. cordatotriloba, whereas crosses between sympatric I. cordatotriloba and I. lacunosa are more successful than crosses between allopatric I. cordatotriloba and I. lacunosa. This difference in compatibility appears to reflect an asymmetric decrease in the strength of the barrier to seed set in sympatric I. cordatotriloba, which could be caused by I. lacunosa alleles that have introgressed into I. cordatotriloba. We further demonstrated that changes to sympatric I. cordatotriloba have decreased its ability to produce seeds with allopatric populations of the same species, in line with our second prediction. Thus, in a manner analogous to cascade reinforcement, we suggest that introgression associated with hybridization not only influences between-species isolation but can also contribute to isolation within a species.

Methods

These data were generated by growing morning glory plants from multiple populations of two species in a common environment and making crosses between the individuals (see Ostevik et al. 2020 for details). We recorded whether crosses produced a fruit. In cases where a fruit was produced, we further recorded the weight of the fruit, the number of seeds inside the fruit, and the weight of those seeds. The data have been lightly filtered to remove unreliable crosses (e.g., cases in which the plant died between pollination and fruit set and cases in which the flower was damaged in some way). 

Funding

National Science Foundation, Award: DEB 1542387

National Science Foundation, Award: DEB 1501954

National Science and Engineering Research Council of Canada, Award: 516658