Data from: Resolving the Northern Hemisphere source region for the long-distance dispersal event that gave rise to the South American endemic dung moss Tetraplodon fuegianus
Lewis, Lily, University of Connecticut
Biersma, Elisabeth M., University of Cambridge
Carey, Sarah B., University of Florida
Holsinger, Kent, University of Connecticut
McDaniel, Stuart F., University of Florida
Rozzi, Ricardo, University of Chile
Goffinet, Bernard, University of Connecticut
Lewis, Lily R., University of Connecticut
Published Oct 18, 2018 on Dryad.
Cite this dataset
Lewis, Lily et al. (2018). Data from: Resolving the Northern Hemisphere source region for the long-distance dispersal event that gave rise to the South American endemic dung moss Tetraplodon fuegianus [Dataset]. Dryad. https://doi.org/10.5061/dryad.2cs4v
Premise of the study—American bipolar plant distributions characterize taxa at various taxonomic ranks but are most common in the bryophytes at infraspecific and infrageneric levels. A previous study on the bipolar disjunction in the dung moss genus Tetraplodon found that direct long-distance dispersal from North to South in the Miocene - Pleistocene accounted for the origin of the Southern American endemic Tetraplodon fuegianus, congruent with other molecular studies on bipolar bryophytes. The previous study, however, remained inconclusive regarding a specific Northern Hemisphere source region for the trans-equatorial dispersal event that gave rise to T. fuegianus.
Methods—To estimate spatial genetic structure and phylogeographic relationships within the bipolar lineage of Tetraplodon, which includes T. fuegianus, we analyzed thousands of Restriction-site Associated DNA (RADseq) loci and single nucleotide polymorphisms using Bayesian individual assignment and maximum likelihood and coalescent model based phylogenetic approaches.
Key results—Northwestern North America is the most likely source of the recent ancestor to T. fuegianus.
Conclusions—Tetraplodon fuegianus, which marks the southernmost populations in the bipolar lineage of Tetraplodon, arose following a single long-distance dispersal event involving a T. mnioides lineage that is now rare in the Northern Hemisphere and potentially restricted to the Pacific Northwest region of North America. Furthermore, gene flow between sympatric lineages of Tetraplodon mnioides in the Northern Hemisphere is limited, possibly due to high rates of selfing or reproductive isolation.
Aligned supermatrices & SNP matrices
Add data sets referred to in Table 1 and Table 2 are included. Supermatrices are in nexus format and unlinked SNP files are in .str format, and include sample assignment information based on RAxML analyses (see Figure 1). Sample assignment information should not be used to inform STRUCTURE analyses however, but to help with graphical display and order of samples. All data files were produced in PyRAD v. 2.17. (Eaton 2014) Details of data file generation can be found in the main text and SI of the associated paper.
demultiplexed & trimmed reads for all samples (n=81)
Compressed package with demultiplexed and trimmed RADseq data for 81 samples (i.e. 81 .fastq.gz files representing all samples listed in Appendix S1). Demultiplexing and trimming was done in PyRAD v. 2.17 (Eaton 2014). Reads were de-multiplexed allowing for a maximum of two mismatches in each eight bp barcode, and restriction enzyme cut sites and adapter sequences were removed. .fastq.gz files are named as such: Sample ID-Collection#.fastq.gz. For example, Nepal_1_1101.fastq.gz is for the sample identified as "Nepal_1" in the associated publication, which was also assigned collection number 1101. The sample ID provides information on the general collection locality.