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Data from: Solanum pennellii (LA5240) backcross inbred lines (BILs) for high resolution mapping in tomato

Torgeman, Shai1; Zamir, Dani1

Published Jan 17, 2024 on Dryad. https://doi.org/10.5061/dryad.2fqz612wx

Data files

Jan 17, 2024 version files 29.70 MB

Abstract

Wild species are an invaluable source of new traits for crop improvement. Over the years the tomato community bred cultivated lines that carry introgressions from different species of the tomato tribe to facilitate trait discovery and mapping. The next phase in such projects is to find the genes that drive the identified phenotypes. This can be achieved by genotyping a few thousand individuals resulting in fine-mapping that can potentially identify the causative gene. To couple trait discovery and fine mapping we are presenting large, recombination-rich, Backcross Inbred Line (BIL) populations involving an unexplored accession of the wild, green-fruited species Solanum pennellii (LA5240; the Lost Accession) with two modern tomato inbreds: LEA, determinate, and TOP indeterminate. The LEA and TOP BILs are in BC2F6-8 generation and include 1,400 and 500 lines respectively. The BILs were genotyped with ~5,000 SPET markers, showing that in the euchromatic regions there was one recombinant every 17-18 Kb while in the heterochromatin a recombinant every 600-700 Kb (TOP and LEA respectively). To gain perspective on the topography of recombination we compared five independent members of the self-pruning gene family with their respective neighboring genes; based on PCR markers, in all cases we found recombinants. Further mapping analysis of two known morphological mutations that segregated in the BILs (Self-pruning and Hair), showed that the maximal delimited intervals were 73 Kb and 210 Kb respectively and included the known causative genes. The LOST_BILs provide a solid framework to study traits derived from a tolerant wild tomato.