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Species delimitation, classical taxonomy, and genome skimming: a review of the ground beetle genus Lionepha (Coleoptera: Carabidae)

Citation

Maddison, David; Sproul, John (2020), Species delimitation, classical taxonomy, and genome skimming: a review of the ground beetle genus Lionepha (Coleoptera: Carabidae), Dryad, Dataset, https://doi.org/10.5061/dryad.2jm63xsjq

Abstract

The western North American genus Lionepha is shown to contain at least 11 species through a combination of eight-gene species delimitation analyses and morphological study. In order to confirm the names of several species, we sequence DNA of primary types of several names, including a LeConte lectotype collected in the 1850s, using next-generation sequencing. We examine chromosomes of eight species, and show that all have 12 pairs of autosomes and an X0/XX sex-chromosome system. The following species are described as new: Lionepha australerasaL. kavanaughiL. lindrothi, and L. tuulukwa. The name Lionepha erasa is shown to belong to a relatively rare, western species ranging from Oregon through Alaska; the common, widespread species previously known as Lionepha erasa now takes the name L. probataBembidion lindrothellusB. chintimini, and B. lummi are synonymized with L. erasa. We provide tools to identify specimens to species, including illustrations and diagnoses.

Methods

DNA was sequenced using both PCR/Sanger sequencing and Illumina sequencing.  Phylogenetic trees included in the submission were inferred using maximum likelihood analyses in IQ-TREE.

Usage Notes

This file is a NEXUS file which can be read by any NEXUS-compliant program, although it is set up for use in Mesquite.

Funding

National Science Foundation, Award: DEB-1258220