Endosymbionts facilitate rapid evolution in a polyphagous herbivore
Lenhart, Paul; White, Jennifer (2020), Endosymbionts facilitate rapid evolution in a polyphagous herbivore, Dryad, Dataset, https://doi.org/10.5061/dryad.2jm63xsmn
All replicate aphid clones derived from a single Arsenophonus infected female, LW, originally described in Wagner et al. 2015 Functional Ecology 29: 1402-1410. The metadata for the accession and the curing process used to generate the Arsenophonus- isoline are described in that paper. Twelve replicate populations were initiated, each with 20 Ars+ and 20 Ars- aphids at time 0. Half of these populations (1-6) were caged on fava (Vicia faba) and half (7-12) on locust (Robinia pseudoacacia).
Every two weeks (until week 16) one subset of 20 aphids per population were transferred to a fresh plant of the same species, and a second subset was transferred to 95% ethanol and had their DNA extracted. Tab1 provides the data associated with each individual extraction. Extraction codes (column E) indicate the population (column A) and individual specimen (column C). Each extraction was checked for the presence (column F) or absence (column G) of Arsenophonus via diagnostic PCR for a segment of the Arsenophonus 23S gene, and were checked for extraction quality via diagnostic PCR for the aphid COI gene. Any extractions that did not yield COI product were considered failed extractions (column H). Each week's (column D) set of aphids per population represent 20 new aphids; aphid numbers (Column C) were randomly assigned and bear no particular significance, and no relationship to one another across populations, treatments or weeks.
U.S. Department of Agriculture, Award: 2014-67013-21576
U.S. Department of Agriculture, Award: Hatch no. 0224651