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Data from: Chicken gut microbiome members limit the spread of an antimicrobial resistance plasmid in Escherichia coli

Citation

Duxbury, Sarah et al. (2021), Data from: Chicken gut microbiome members limit the spread of an antimicrobial resistance plasmid in Escherichia coli, Dryad, Dataset, https://doi.org/10.5061/dryad.2jm63xsp7

Abstract

Plasmid-mediated antimicrobial resistance is a major contributor to the spread of resistance genes within bacterial communities. Successful plasmid spread depends upon a balance between plasmid fitness effects on the host and rates of horizontal transmission. While these key parameters are readily quantified in vitro, the influence of interactions with other microbiome members is largely unknown. Here, we investigated the influence of three genera of lactic acid bacteria (LAB) derived from the chicken gastrointestinal microbiome on the spread of an epidemic narrow-range ESBL resistance plasmid, IncI1 carrying blaCTX-M-1, in mixed cultures of isogenic Escherichia coli strains. Secreted products of LAB decreased E. coli growth rates in a genus-specific manner but did not affect plasmid transfer rates. Importantly, we quantified plasmid transfer rates by controlling for density-dependent mating opportunities. Parametrization of a mathematical model with our in vitro estimates illustrated that small fitness costs of plasmid carriage may tip the balance towards plasmid loss under growth conditions in the gastrointestinal tract. This work shows that microbial interactions can influence plasmid success and provides an experimental-theoretical framework for further study of plasmid transfer in a microbiome context.

Usage Notes

ReadMe files for raw and processed data and code, per figure:

README_Fig1a

README_Fig1b_SupplFig2

README_Fig1c

README_Fig1d

README_SupplFig1

Funding

ZonMw, Award: 541001005