Testosterone is strongly associated with the annual development of antlers in cervids, but. endocrine research on wild, freely breeding ungulates is often done without repeated capture of known-aged individuals. As a result, our knowledge on how testosterone fluctuates over the course of a lifetime and variation in lifetime patterns among individuals is limited. We investigated patterns of testosterone in a freely breeding population of white-tailed deer (Odocoileus virginianus) in Alabama, USA that breeds in January. Testosterone peaked during the height of the breeding season, despite this period occurring approximately two months later than in most temperate-region, white-tailed deer populations. Age-related differences in testosterone were only prevalent during the breeding season, with bucks ≥3.5 years old having greater testosterone (853 ng/dl ±96 SE; P = 0.012) than bucks 1.5–2.5 years old (364 ng/dl ±100 SE). Additionally, an individual’s testosterone level as a yearling was not positively associated with their lifetime maximum testosterone level (P = 0.583), and an individual’s mean testosterone level was positively associated with lifetime testosterone variation (P < 0.001). To our knowledge our study is one of the first to assess how testosterone early in life might relate to individual testosterone later in life. We believe these data provide insight into lifetime hormonal patterns in cervids, and that these patterns may indicate intraspecific variation of lifetime reproductive strategies.