Data for: Neuroendocrine gene expression coupling of interoceptive bacterial food cues to foraging behavior of C. elegans
Data files
Jan 11, 2024 version files 311.39 KB
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Figure_1D.xlsx
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Figure_5D.xlsx
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Genotyping_Primers.xlsx
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README.md
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Abstract
Animal internal state is modulated by nutrient intake, resulting in behavioral responses to changing food conditions. The neural mechanisms by which internal states are generated and maintained are not well understood. Here, we show that in the nematode Caenorhabditis elegans, distinct cues from bacterial food – interoceptive signals from the ingestion of bacteria and gustatory molecules sensed from nearby bacteria – act antagonistically on the expression of the neuroendocrine TGF-beta ligand DAF-7 from the ASJ pair of sensory neurons to modulate foraging behavior. A positive-feedback loop dependent on the expression of daf-7 from the ASJ neurons acts to promote transitions between roaming and dwelling foraging states and influence the persistence of roaming states. SCD-2, the C. elegans ortholog of mammalian Anaplastic Lymphoma Kinase (ALK), which has been implicated in the central control of metabolism of mammals, functions in the AIA interneurons to regulate foraging behavior and cell-non-autonomously control the expression of DAF-7 from the ASJ neurons. Our data establish how a dynamic neuroendocrine daf-7 expression feedback loop regulated by SCD-2 functions to couple sensing and ingestion of bacterial food to foraging behavior. We further suggest that this neuroendocrine feedback loop underlies previously characterized exploratory behaviors in C. elegans. Our data suggest that the expression of daf-7 from the ASJ neurons contributes to and is correlated with an internal state of “unmet need” that regulates exploratory foraging behavior in response to bacterial cues in diverse physiological contexts.
README: Data for: Neuroendocrine gene expression coupling of interoceptive bacterial food cues to foraging behavior of C. elegans
https://doi.org/10.5061/dryad.2ngf1vhwn
All data is from fluorescence microscopy, C. elegans activity assays, or roaming and dwelling assays.
Description of the data and file structure
Data is uploaded as .xlsx files and labeled by the figure number in the eLife manuscript.
- Figures 1D, 1E, 1F, 1G, 1H, 3B, 3D, 4A, 4B, 4D, 5A, and 5C represent quantified pdaf-7::gfp expression in ASJ. Data consists of lists of individual maximum GFP fluorescence values in ASJ normalized to expression time for individual animals. Quantification was performed in FIJI and is in arbitrary brightness units.
- Figures 2A right, 2C right, 4E right, 4G right, and 4J right are data from exploration assays. The exploration assay was performed loosely as previously described with several modifications (Flavell et al., 2013). Thirty-five millimeter NGM plates with no peptone were seeded with 500 µL OP50 grown overnight in LB so that the lawn covered the entire surface of the plate. On the day of the assay, one day-one adult animal was placed on the plate and allowed to explore for two hours before being removed. The plate was then superimposed on a grid of 3.5 mm squares and the number of squares crossed by the C. elegans tracks was manually counted. The data files represent lists of numbers of squares entered for individual animals of the corresponding genotypes.
- Figures 2A left, 2B, 2C left, 3A, 3C, 4E Left, 4G left, 4I, 4J, 5B, and 5D are data from roaming and dwelling assays. Data consists of lists of fraction of time roaming for individual animals calculated with WormLab.
- Figures 2F, 4F, and 4H represent durations of individual roaming or dwelling states (seconds). Each value represents an individual state experienced by an individual animal.
- Also included is a table of genotyping primer sequences for alleles first reported in this manuscript.
More details can be found in the manuscript materials and methods.