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The role of tetradecane in the identification of host plants by the pest bugs Apolygus lucorum and Adelphocoris suturalis

Citation

Yin, Haichen (2022), The role of tetradecane in the identification of host plants by the pest bugs Apolygus lucorum and Adelphocoris suturalis , Dryad, Dataset, https://doi.org/10.5061/dryad.2rbnzs7qt

Abstract

Apolygus lucorum and Adelphocoris suturalis are considered serious pests to many cultures in China. Safe alternatives are needed to manage these mirid pests. The current study identified compounds from  volatiles of the ethanol extracts of Phaseolus vulgaris pods and the other 10 common hosts. GCMS detection showed that tetradecane, 2-propyl-1-pentanol, and dodecanal were included in these volatiles and   tetradecane was present in all the volatiles.

Methods

About 10 mL of any given extract was added to a glass jar (35 × 20 cm – height and diameter) provided with an airflow inlet and outletat the top. An adsorption column was attached to the outlet, while activated carbon filter (air flow rate: 600 L/min) was attached to the inlet. After collecting for 8 h, the adsorption column was eluted with chromatographically-pure hexane for GC-MS detection of samples.

Gas chromatograph settings included an Rtx-5 MS chromatographic column (30 m. 0.25 mm; 0.25 µm), injector temperature set to 250°C, the carrier gas was helium, and injections were made on splitless mode. The oven temperature program was set to three steps: (i) 40°C for 1 min; (ii) increased to 130°C at the rate of 4°C/min, maintaining for 5 min; (iii) increased to 250°C at the rate of 10°C/min and maintaining for 5 min. Mass spectrometer has its ion source temperature set to 250°C, and scanning speed at 2500, with a 0.3 s interval.

Mass spectra were compared with standards in the Labsolution software to predict the chemical structure of the main volatiles in P. vulgaris pods extracts.

Usage Notes

Mass spectra were compared with standards in the Labsolution software to predict the chemical structure and all data analyses were conducted using SPSS software

Funding

National Natural Science Foundation of China, Award: 32001966