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Dryad

Saw Kill river (NY, USA) metagenomics and environmental variables

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Sep 19, 2022 version files 49.61 MB
Nov 09, 2022 version files 49.64 MB

Abstract

Microbial community structure and diversity in waterways are altered by wastewater treatment plant (WWTP) discharge which can introduce human-associated microbes, antimicrobial resistance (AMR), and significantly change environmental variables. To better understand these interactions, we investigated the impact of the Bard College WWTP on microbial communities collected from the surface water and sediment from four sites, two sites above and two sites below the Bard College outflow, as well as the outflow itself, this was performed over a period of five months. We measured physico-chemical parameters such as temperature, turbidity, conductivity, dissolved oxygen, and salinity as well as the bioindicators Escherichia coli, total coliforms, and Enterococcus sp. concentration, endotoxins, the intI1 gene marker, and the total bacterial abundance through 16S rRNA gene.