Skip to main content
Dryad logo

Stochastic cell-cycle entry and cell-state-dependent fate outputs of injury-reactivated tectal radial glia in zebrafish

Citation

Yu, Shuguang; He, Jie (2019), Stochastic cell-cycle entry and cell-state-dependent fate outputs of injury-reactivated tectal radial glia in zebrafish, Dryad, Dataset, https://doi.org/10.5061/dryad.31t3425

Abstract

Gliosis defined as reactive changes of resident glia is the primary response of the central nervous system (CNS) to trauma. The proliferation and fate controls of injury-reactivated glia are essential but remain largely unexplored. In zebrafish optic tectum, we found that stab injury drove a subset of radial glia (RG) into the cell cycle, and surprisingly, proliferative RG responding to sequential injuries of the same site were distinct but overlapping, which was in agreement with stochastic cell-cycle entry. Single-cell RNA sequencing analysis and functional assays further revealed the involvement of Notch/Delta lateral inhibition in this stochastic cell-cycle entry. Furthermore, the long-term clonal analysis showed that proliferative RG were largely gliogenic. Notch inhibition of reactive RG, not dormant and proliferative RG, resulted in an increased production of neurons, which were short-lived. Our findings gain new insights into the proliferation and fate controls of injury-reactivated CNS glia in zebrafish.

Usage Notes

Funding

Chinese Academy of Sciences, Award: Strategy Priority Research Program XDB32000000

National Natural Science Foundation of China, Award: 31471042

Shanghai Municipal Science and Technology Major Project, Award: 2018SHZDZX05

Shanghai Basic Research Field Project, Award: 18JC1410100

China Thousand Talents Program