Data from: Chikungunya virus impacts the diversity of symbiotic bacteria in mosquito vector
Data files
Jan 24, 2012 version files 36.27 KB
-
Kinetics_CHIKV.xlsx
12.29 KB
-
README_for_Kinetics_CHIKV.txt
645 B
-
README_for_Relative_density_bacteria_Ae. albopictus_ALPROV.txt
1.18 KB
-
README_for_Relative_Intensity_Probes discriminating infected from noninfected.txt
815 B
-
Relative_density_bacteria_Ae. albopictus_ALPROV.xlsx
14.92 KB
-
Relative_Intensity_Probes discriminating infected from noninfected.csv
6.41 KB
Abstract
Mosquitoes transmit numerous arboviruses including dengue and chikungunya virus (CHIKV). In recent years, mosquito species Aedes albopictus has expanded in the Indian Ocean region and was the principal vector of chikungunya outbreaks in La Reunion and neighbouring islands in 2005 and 2006. Vector-associated bacteria have recently been found to interact with transmitted pathogens. For instance, Wolbachia modulates the replication of viruses or parasites. However there has been no systematic evaluation of the diversity of the entire bacterial populations within mosquito individuals particularly in relation to virus invasion. Here, we investigated the effect of CHIKV infection on the whole bacterial community of Ae. albopictus. Taxonomic microarrays and quantitative PCR showed that members of Alpha-, and Gamma-proteobacteria phyla, as well as Bacteroidetes, responded to CHIKV infection. The abundance of bacteria from the Enterobacteriaceae family increased with CHIKV infection whereas the abundance of known insect endosymbionts like Wolbachia and Blattabacterium decreased. Our results clearly link pathogen propagation with changes in the dynamics of the bacterial community suggesting that cooperation or competition occurs within the host, which may in turn affects mosquito traits like vector competence.