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Ancient sedimentary plant DNA and pollen dataset from Bolshoe Toko Lake, southeastern Siberia

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Apr 14, 2021 version files 1.36 GB

Abstract

Here we provide a dataset on genetic and pollen plant diversity retrieved from sedimentary ancient DNA (sedaDNA) of the Bolshoe Toko lake from southeastern Siberia. Our dataset encompasses sedaDNA sequence data of 54 lake sediment samples and pollen grain counts of 67 lake sediments samples. We used a PCR-based metabarcoding approach combined with Next-Generation Sequencing to assess the past, local plant diversity around the analysed lake localities. As a plant specific metabarcode we applied the established chloroplastidal P6 loop trnL marker for plant diversity assessment. PCR products were sequenced on one Illumina sequencing run (HUA-9). Pollen and non-pollen palynomorphs (NPP) were identified using a light microscope using pollen atlases and pollen reference collections at the Arctic and Antarctic Research Institute (Sankt-Petersburg) and the Alfred Wegener Institute.