Biomedical device-associated infection(BAI) and lack of osseointegration are the main causes of implant failure. Therefore, it is imperative for implant not only to depress microbial activity and biofilm colonization but also to prompt osteoblast functions and osseointegration. As part of the coating development for implants, the interest of in vitro studies on the interaction between implant substrate morphology and biological performances is growing. In this study, by harnessing the adhesion and reactivity of bioinspired polydopamine, nano-silver were successfully anchored onto micro/nanoporous as well as smooth titanium surfaces to analyze the effect of substrate morphology on biological performances of the coatings. Compared with smooth surface, small size of nano-Ag and high Ag content was found on the micro/nanoporous surface. More mineralization happened on the coating on micro/nanoporous structure than on smooth surface, which led to a more rapid decrease of Ag release from micro/nanoporous surface. Antimicrobial tests indicated that both surfaces with resulting coating inhibit microbial colonization on them and growth around them, indicating that the coating eliminates the shortcoming of porous structure which render the implant extremely susceptible to BAI. Besides, the multiple osteoblast responses of nano-Ag-loaded dopamine coatings on both surfaces, i.e. attachment, proliferation and differentiation, have deteriorated, however the mineralized surfaces of these coatings stimulated osteoblast proliferation and differentiation, especially for the micro/nanoporous surface. So the resulting coatings on micro/nanoporous substratum may not only reduce the risk of infection but also facilitate mineralization during the early post-operative period and then promote osseointegration due to good osteoblast-biocompatibility of the mineralized surface. These results highlight the influence of the substrate morphology on the biological performances of implant coating.