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Data for: Tunable self-cleaving ribozymes for modulating gene expression in eukaryotic systems

Citation

Jacobsen, Thomas et al. (2020), Data for: Tunable self-cleaving ribozymes for modulating gene expression in eukaryotic systems, Dryad, Dataset, https://doi.org/10.5061/dryad.3j9kd51dz

Abstract

Advancements in the field of synthetic biology have been possible due to the development of genetic tools that are able to regulate gene expression. However, the current toolbox of gene regulatory tools for eukaryotic systems have been outpaced by those developed for simple, single-celled systems. Here, we engineered a set of gene regulatory tools by combining self-cleaving ribozymes with various upstream competing sequences that were designed to disrupt ribozyme self-cleavage. As a proof-of-concept, we were able to modulate GFP expression in mammalian cells, and then showed the feasibility of these tools in Drosophila embryos. For each system, the fold-reduction of gene expression was influenced by the location of the self-cleaving ribozyme/upstream competing sequence (i.e. 5′ vs. 3′ untranslated region) and the competing sequence used. Together, this work provides a set of genetic tools that can be used to tune gene expression across various eukaryotic systems.

Methods

Confocal microscopy

Usage Notes

Refer to the manuscript (open access) for name designations.

Funding

National Science Foundation, Award: MCB-1413044

U.S. Department of Education, Award: 2P40OD010949