Skip to main content
Dryad logo

Data from: The complex synaptic pathways onto a looming-detector neuron revealed using serial block-face scanning electron microscopy (SBEM)

Citation

Leitinger, Gerd et al. (2021), Data from: The complex synaptic pathways onto a looming-detector neuron revealed using serial block-face scanning electron microscopy (SBEM), Dryad, Dataset, https://doi.org/10.5061/dryad.3j9kd51hc

Abstract

The locust’s lobula giant movement detector 1 (LGMD1) looming detector pathway is part of the compound eye visual system and enables the animals to reliably detect collisions. Trans-medullary afferent neurons are considered key players in this pathway. Thousands of these neurons connect the second visual neuropile region, or medulla, with the third neuropile region, or lobula complex. In the lobula complex they are in synaptic contact with the LGMD1, which forms a dendritic tree in the outer region of the lobula complex neuropile. In order to describe their anatomy and connectivity patterns with other upstream neurons of the LGMD1, we used serial block-face scanning electron microscopy. We thus produced serial electron micrographs spanning from the dendritic tree in the outer lobula complex to the origin of the trans-medullary afferent neurons in the medulla. Starting from the LGMD1, we segmented and 3D-reconstructed entire trans-medullary afferents, as well as connecting neurons and other trans-medullary neurons nearby. This study was based on two datasets from different locusts of fourth instar. Here we provide the raw data for this study as .tiff stacks.

Methods

The optic lobe of 2 locusts (Locusta migratoria) of fourth instar were fixed, dehydrated, block stained, and embedded in TAAB epoxy resin. A block face was trimmed through the outer lobula. Serial scans were made from the outer lobula, through the outer chiasm, into the medulla. An in-situ ultra-microtome 3View (Gatan, Inc., Pleasanton, CA, USA), mounted in the chamber of an environmental scanning electron microscope ESEM Quanta 600 FEG (FEI, Eindhoven, Netherlands) at the Graz University of Technology was used for image acquisition. Microscope and imaging conditions: 3kV acceleration voltage, water vapour with a pressure of 20 Pa, spot size 3.2, dwell time of 12.5 µs. Backscattered electron detector (BSED; Gatan, Inc.). 

Usage Notes

Wernitznig_2021_Locust_L4_Lobula_To_Medulla_Dataset01

Wernitznig_2021_Locust_L4_Lobula_To_Medulla_Dataset02

These datasets includes the serial sections used in the following paper: Wernitznig, S; Zankel, A; Bock, E; Gütl, D; Hobusch, U; Nicolic, M; Pargger, L; Pritz, E; Radulović, S; Sele, M; Summerauer, S; Pölt, P; Rind, FC,  and G. Leitinger. The complex synaptic pathways onto a looming-detector neuron revealed using serial block-face scanning electron microscopy (SBEM) (J. Comp Neurol, doi: 10.1002/cne.25227). Each dataset starts in the outer lobula complex of a locust of fourth instar, and covers the outer lobula, the outer optic chiasm, and the medulla. Voxel size 10 x 10 x 40-50 nm; section. The TmA neuron is kept in the center of the field of view, so the field of view was moved in a lateral direction between the stacks. Image size in dataset one was 25 x 25 µm, for dataset 2, it was 30 x 30 µm in the lobula complex, when nearing the second optic chiasm, and 20 x 40 µm, within the chiasm, 25 x 25 µm, and in the medulla either 30 x 30, or 35 x 35 µm.

Funding

Austrian Science Fund, Award: P 32058

Austrian Science Fund, Award: P 32376

Land Steiermark, Styrian Provincial Government, Award: HTI:SmApp 2012

Land Steiermark, Styrian Provincial Government, Award: HTI:SmApp 2012