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Effects of temperature treatments on cytosine-methylation profiles of diploid and tetraploid plants of the alpine species Ranunculus kuepferi (Ranunculaceae)

Citation

Syngelaki, Eleni; Schinkel, Christoph C-F.; Klatt, Simone; Hörandl, Elvira (2021), Effects of temperature treatments on cytosine-methylation profiles of diploid and tetraploid plants of the alpine species Ranunculus kuepferi (Ranunculaceae), Dryad, Dataset, https://doi.org/10.5061/dryad.3n5tb2rcq

Abstract

The current dataset refers to the DNA methylation patterns of diploid and tetraploid individuals of Ranunculus kuepferi, obtained with the method of methylation-sensitive AFLPs (MS-AFLPs).

The individuals of Ranunculus kuepferi were collected from several locations throughout the distribution of the species in the Alps, transferred to the old Botanical Garden of Göttingen and placed into two climate chambers MC1000E (Snijders Scientific, Tilburg, Netherlands), where the temperature treatment experiments took place. In the first chamber a cold treatment was applied (+7°C day/+2°C night; frost treatment: -1°C cold shocks for three nights per week), while in the second chamber a warm treatment was applied (+15° day/+10°C night).

The plants were shifted from one treatment to the other one after the end of the 2016 flowering period and leaf material was collected during the flowering period of 2016 and 2017. This material went through the respective lab procedures in order to obtain the genome-wide patterns of epigenetic variation via MS-AFLPs.

The analysis of the electropherograms was conducted with Peakscanner v.2 and fragment scoring was performed with RawGeno 2.0-1 R package. These fragment scoring binary matrices are presented here.

Funding

Deutsche Forschungsgemeinschaft