Data from: Multiple strong postmating and intrinsic postzygotic reproductive barriers isolate florally diverse species of Jaltomata (Solanaceae)
Kostyun, Jamie Lynn, Indiana University Bloomington
Moyle, Leonie Clare, Indiana University Bloomington
Published Apr 03, 2017 on Dryad.
Cite this dataset
Kostyun, Jamie Lynn; Moyle, Leonie Clare (2017). Data from: Multiple strong postmating and intrinsic postzygotic reproductive barriers isolate florally diverse species of Jaltomata (Solanaceae) [Dataset]. Dryad. https://doi.org/10.5061/dryad.3vr00
Divergence in phenotypic traits often contributes to premating isolation between lineages, but could also promote isolation at postmating stages. Phenotypic differences could directly result in mechanical isolation or hybrids with maladapted traits; alternatively, when alleles controlling these trait differences pleiotropically affect other components of development, differentiation could indirectly produce genetic incompatibilities in hybrids. Here, we determined the strength of 9 postmating and intrinsic postzygotic reproductive barriers among 10 species of Jaltomata (Solanaceae), including species with highly divergent floral traits. To evaluate the relative importance of floral trait diversification on the strength of these postmating barriers, we assessed their relationship to floral divergence, genetic distance, geographical context, and ecological differences, using conventional tests and a new linear mixed modeling approach. Despite close evolutionary relationships, all species pairs showed moderate to strong isolation. Nonetheless, floral trait divergence was not a consistent predictor of the strength of isolation; instead this was best explained by genetic distance, although we found evidence for mechanical isolation in one species, and an overall positive relationship between floral trait divergence and fruit set isolation across species pairs. Overall, our data indicate that intrinsic postzygotic isolation is more strongly associated with genome-wide genetic differentiation, rather than floral divergence.
Crossing Data used to Calculate Isolation Values
This file contains data obtained from controlled hybridizations (crosses) in the greenhouse, among different Jaltomata
species and their F1 hybrids. These data were used to calculate measures of reproductive isolation for the following stages:
fruit set, fruit size, seed set, viable seed set, F1 fruit set, F1 fruit size, and F1 seed set.
Measured Floral Color Attributes used to Calculate Floral Distances Metrics
This file contains measures of color attributes for petal and nectar color. For each color trait, images were standardized
for lighting, and then different color attributes were measured in ImageJ. These data were used to calculate floral distance metrics.
Measured Floral Morph Traits used to Calculated Floral Distance Metrics
This file contains measures of floral organs and nectar volume, which were collected on fresh mature flowers. These data were used to calculate floral distance metrics.
Pollen Growth Data used to Calculate Pollen-Pistil Isolation
This file contains data obtained from pollen growth assays, which were used to determine extent of pollen-pistil isolation. It includes species pairs, individuals used, pollination and style collection dates, and pollen tube length information.
Pollen Viability Data used to Calculate F1 Hybrid Pollen Isolation
This file contains pollen count data, which were used to determine F1 pollen fertility isolation. Pollen counts were obtained using aniline-blue stain.
Loci used to Estimate Genetic Distance among Species
This is a compressed file containing alignments for the 60 loci used to estimate genetic distance among Jaltomata species.
Short Description File
This is a readme file containing descriptions of the included data files.
National Science Foundation, Award: DEB 1136707, DEB 1342962, DEB 1601078