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DNA sequences employed in systematics of Lobelioideae (Campanulaceae): Review, phylogenetic and biogeographic analyses

Citation

Kagame, Samuel; Chen, Lingyun; Gichira, Andrew; Wang, Qingfeng (2021), DNA sequences employed in systematics of Lobelioideae (Campanulaceae): Review, phylogenetic and biogeographic analyses, Dryad, Dataset, https://doi.org/10.5061/dryad.3xsj3txfw

Abstract

Lobelioideae, the largest subfamily within Campanulaceae, includes 33 genera and approximately1200 species. It is characterized by resupinate flowers with zygomorphic corollas and connate anthers and widely distributed across the world. The systematics of Lobelioideae has been quite challenging over the years, with different scholars postulating varying theories. To outline major progress and highlight the existing systematic problems in Lobelioideae, we conducted a literature review on this subfamily. Additionally, we conducted phylogenetic and biogeographic analyses for Lobelioideae using plastids and internal transcribed spacer regions. We found that former studies have reached agreement on the southern African origin of Lobelioideae, herbaceous habit and Asian origin of giant lobelioids, the convergent evolution of giant rosette lobelioids, and lastly, the multiple cosmopolitan and independent radiation of lobelioids in Africa, Pacific Basin, and the Hawaiian Islands. Also, Apetahia Baill., Sclerotheca A.DC., and Cyanea Gaudich. are paraphyletic, while Lobelia L., Pratia Gaudich., Centropogon C.Presl, Siphocampylus Pohl, and Isotoma Lindl. are polyphyletic. The taxonomy of these genera, especially Lobelia, is particularly quite frustrating. This calls for further reappraisals using both morphological and molecular data.

Methods

Nineteen loci were obtained, that is, eighteen plastid gene loci (atpB-rbcL spacer, atpB, atpF, atpF-atpH spacer, atpH, matK, ndhF, psbA-trnH spacer, psbA-trnK spacer, petD, rbcL, rpoC1, trnL-trnF spacer, trnT-trnL spacer, trnV-trnK spacer, trnK-matK spacer, rpl32-ndhF spacer, rpl16) and one nuclear gene, internal transcribed spacer (ITS). These sequences were generated using the NCBI ENTREZ UTILITY program (Accessed 1ST April 2020) and double-checked manually at the GenBank database. Additionally, almost all available Campanulaceae complete plastid genomes were manually accessed from the GenBank. The respective plastid regions were extracted using NCBI BLASTN v. 2.9.0+ with default settings.

Usage Notes

See ReadMe.txt file

Funding

National Natural Science Foundation of China, Award: 31670226

CAS “The Belt and Road” Master Fellowship Programme, Award: No.2018BRF052

CAS “The Belt and Road” Master Fellowship Programme, Award: No.2018BRF052