Data from: A population genetics perspective on the evolutionary histories of three clonal, endemic, and dominant grass species of the Qinghai-Tibet Plateau: Orinus (Poaceae)
Liu, Yuping, Qinghai Normal University
Harris, A.J., Oberlin College
Gao, Qingbo, Chinese Academy of Sciences
Su, Xu, Qinghai Normal University
Ren, Zhumei, Shanxi University
Published Apr 29, 2019 on Dryad.
Cite this dataset
Liu, Yuping et al. (2019). Data from: A population genetics perspective on the evolutionary histories of three clonal, endemic, and dominant grass species of the Qinghai-Tibet Plateau: Orinus (Poaceae) [Dataset]. Dryad. https://doi.org/10.5061/dryad.403j5s4
We performed analyses of amplified fragment length polymorphism (AFLP) in order to characterize the evolutionary history of Orinus according to its population genetic structure, to investigate putative hybrid origins of O. intermedius, and to provide additional insights on relationships among species. The genus Orinus comprises three clonal grasses that are dominant species within xeric alpine grasslands of the Qinghai-Tibet Plateau (QTP). Here, we used eight selectively-obtained primer pairs of EcoRI/MseI to perform amplifications in 231 individuals of Orinus representing 48 populations and all three species. We compared our resulting data to genetic models of hybridization using a Bayesian algorithm within NewHybrids software. We determined that genetic variation in Orinus was 56.65% within populations while the among-species component was 30.04% using standard population genetics statistics. Nevertheless, we detected that species of Orinus were clustered into three highly distinct genetic groups corresponding to classic species identities. Our results suggest that there is some introgression among species. However, O. intermedius likely derives from a common ancestor with O. kokonoricus and is probably not the result of hybrid speciation between O. kokonoricus and O. thoroldii. We suspect that recent isolation of species of Orinus in allopatry via vicariance may explain the patterns in diversity that we observed, and this is corroborated by a mantel test that showed significant positive correlation between geographic and genetic distance (r = 0.05, P < 0.05). Recent isolation may explain why Orinus differs from many other clonal species by exhibiting the highest diversity within populations rather than among them.
AFLP scoring for sampled individuals of Orinus
The binary (presence/absence) scoring of AFLP bands for sampled individuals of Orinus. The bands were scored in GeneScan 3.1. Within the spreadsheet, the first letter in the sample number in column one refers to the species so that T=O. thoroldii, I=O. intermedius, K=O. kokonoricus, and other numbers and letters yield unique designations. The population numbers in column two correspond with those in Figure 1 in the main text of our publication.
Supplemental File - AFLP scoring -For Submission to Dryad.xlsx