Data from: The selective myosin II inhibitor blebbistatin reversibly eliminates gastrovascular flow and stolon tip pulsations in the colonial hydroid Podocoryna carnea
Data files
Nov 11, 2016 version files 23.12 GB
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Figure 5 time and length.csv
95.98 KB
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Figure 6 time and length.csv
53.70 KB
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Figure 7 time and length.csv
31.70 KB
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Intact rep 6_fig 5_part 1a.zip
940.53 MB
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Intact rep 6_fig 5_part 1b.zip
942.26 MB
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Intact rep 6_fig 5_part 1c.zip
942.59 MB
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Intact rep 6_fig 5_part 1d.zip
337.38 MB
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Intact replicate 1_after reagent.zip
175.68 MB
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Intact replicate 1_prior to reagent.zip
176.49 MB
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Intact replicate 1_recovery.zip
174.28 MB
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Intact replicate 2_after reagent.zip
180.75 MB
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Intact replicate 2_prior to reagent.zip
180.68 MB
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Intact replicate 2_recovery.zip
181.65 MB
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Intact replicate 3_after reagent.zip
171.50 MB
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Intact replicate 3_prior to reagent.zip
169.13 MB
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Intact replicate 3_recovery.zip
171.88 MB
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intact replicate 4 after reagent.zip
159.10 MB
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intact replicate 4 prior to reagent.zip
157.61 MB
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intact replicate 4 recovery.zip
160.18 MB
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Intact replicate 5 after reagent.zip
162.45 MB
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Intact replicate 5 prior to reagent.zip
160.35 MB
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Intact replicate 5 recovery.zip
161.85 MB
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Intact_rep 6_fig 5_pt 2a.zip
933.43 MB
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Intact_rep 6_fig 5_pt 2b.zip
885.47 MB
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Intact_rep 6_fig 5_pt 2c.zip
876.64 MB
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Intact_rep 6_fig 5_pt 2d.zip
899.73 MB
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Intact_rep 6_fig 5_pt 2e.zip
896.64 MB
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Intact_rep 6_fig 5_pt 2f.zip
888.30 MB
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Intact_rep 6_fig 5_pt 2g.zip
893.26 MB
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Intact_rep 6_fig 5_pt 2h.zip
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Intact_rep 6_fig 5_pt 2i.zip
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Intact_rep 6_fig 5_pt 2j.zip
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Isolated rep 1_fig 6_pt 1a.zip
679.23 MB
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Isolated rep 1_fig 6_pt 1b.zip
696.85 MB
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Isolated rep 1_fig 6_pt 2a.zip
960.73 MB
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Isolated rep 1_fig 6_pt 2b.zip
987.14 MB
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Isolated rep 1_fig 6_pt 2c.zip
971.43 MB
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Isolated rep 1_fig 6_pt 2d.zip
579.69 MB
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Isolated replicate 2 after reagent added.zip
191.89 MB
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Isolated replicate 2 prior to reagent.zip
194.27 MB
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Isolated replicate 2 recovery.zip
200.80 MB
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Isolated replicate 3 after reagent.zip
199.24 MB
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Isolated replicate 3 prior to reagent.zip
202.91 MB
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Isolated replicate 3 recovery.zip
197.81 MB
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Latrunculin rep 2_fig 7_pt 1.zip
595.55 MB
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Latrunculin rep 2_fig 7_pt 2.zip
656.01 MB
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Latrunculin replicate 1 after reagent.zip
101.49 MB
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Latrunculin replicate 1 prior to reagent.zip
171.90 MB
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Latrunculin replicate 1 recovery.zip
168.63 MB
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Latrunculin replicate 3 after reagent.zip
107.67 MB
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Latrunculin replicate 3 prior to reagent.zip
179.61 MB
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Latrunculin replicate 3 recovery.zip
161.07 MB
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READ ME.txt
10.39 KB
Abstract
Blebbistatin reversibly disrupted both stolon tip pulsations and gastrovascular flow in the colonial hydroid Podocoryna carnea. Epithelial longitudinal muscles of polyps were unaffected by blebbistatin, as polyps contracted when challenged with a pulse of KCl. Latrunculin B, which sequesters G actin preventing F actin assembly, caused stolons to retract, exposing focal adhesions where the tip epithelial cells adhere to the substratum. These results are consistent with earlier suggestions that non-muscle myosin II provides the motive force for stolon tip pulsations and further suggest that tip oscillations are functionally coupled to hydrorhizal axial muscle contraction.