Numerical data from: Gasotransmitter modulation of hypoglossal motoneuron activity
Citation
Garcia, Alfredo; Browe, Brigitte (2023), Numerical data from: Gasotransmitter modulation of hypoglossal motoneuron activity, Dryad, Dataset, https://doi.org/10.5061/dryad.44j0zpchc
Abstract
Using the rhythmic brainstem slice preparation, which contains the preBötzinger complex (preBötC) and the hypoglossal nucleus, we tested the hypothesis that central HO-2 dysregulation weakens hypoglossal motoneuron output. Disrupting HO-2 activity increased transmission failure as determined by the intermittent inability of the preBötC rhythm to trigger output from the hypoglossal nucleus. Failed transmission was associated with a reduced input-output relationship between the preBötC and the motor nucleus. These network phenomena were related to smaller inspiratory drive currents and reduced intrinsic excitability among hypoglossal neurons. In addition to HO-2, hypoglossal neurons also expressed the CO-regulated H2S producing enzyme cystathionine ϒ-lyase (CSE). H2S abundance was higher in hypoglossal neurons of HO-2 null mice than wild-type controls. Disrupting CSE function normalized transmission in HO-2 null mice and an H2S donor mimicked the effects of HO-2 dysregulation. These findings demonstrate a hitherto uncharacterized modulation of hypoglossal activity through the interaction of HO‑2 and CSE-derived H2S, and supports the perspective that centrally derived HO-2 activity plays an important role regulating upper airway control. This archived dataset contains the numerical data used to generate the figures in the preprint titled: Gasotransmitter Modulation of Hypoglossal Motoneuron Activity by Brigitte Browe, Ying-Jie Peng, Jayasri Nanduri, Nanduri R. Prabhakar, and Alfredo J. Garcia III. This report can be found at https://www.biorxiv.org/content/10.1101/2022.03.23.485481.
Methods
These data were collected from electrophysiological measurements. Data was analyzed posthoc using CLAMPFIT, a part of the pCLAMP software suite (Molecular Devices). Numerical values were generated using spreadsheet calculations in Excel (Microsoft). The resulting values were aggregated in a single summary file for each figure.
Usage notes
Data is archived in a Microsoft Excel format.
Funding
National Heart, Lung, and Blood Institute, Award: P01 HL144454
National Institute of Neurological Disorders and Stroke, Award: R01NS107421
National Heart, Lung, and Blood Institute, Award: R01HL163965
National Institute on Drug Abuse, Award: R01DA057767